RGD Reference Report - Identification of a non-canonical tyrosine-based endocytic motif in an ionotropic receptor. - Rat Genome Database

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Identification of a non-canonical tyrosine-based endocytic motif in an ionotropic receptor.

Authors: Royle, Stephen J  Bobanovic, Laura K  Murrell-Lagnado, Ruth D 
Citation: Royle SJ, etal., J Biol Chem. 2002 Sep 20;277(38):35378-85. doi: 10.1074/jbc.M204844200. Epub 2002 Jul 8.
RGD ID: 13461854
Pubmed: (View Article at PubMed) PMID:12105201
DOI: Full-text: DOI:10.1074/jbc.M204844200

Rapid modulation of the surface number of certain ionotropic receptors is achieved by altering the relative rates of insertion and internalization. These receptors are internalized by a clathrin-mediated pathway; however, a motif that is necessary for endocytosis of ionotropic receptors has not yet been identified. Here, we identified a motif that is required for constitutive and agonist-regulated internalization of the ionotropic P2X(4) receptor. Three amino acids in the C terminus of P2X(4) (Tyr(378), Gly(381), and Leu(382)) compose a non-canonical tyrosine-based sorting signal of the form YXXGL. We found that P2X(4) protein was present in clathrin-coated vesicles isolated from rat brain and that a glutathione S-transferase fusion of the P2X(4) C terminus pulled down the adaptor protein-2 complex from brain extract. Mutation of either the tyrosine-binding pocket of the mu2 subunit of adaptor protein-2 or the YXXGL motif in the receptor C terminus caused a decrease in receptor internalization and a dramatic increase in the surface expression of P2X(4) receptors. The YXXGL motif represents a non-canonical tyrosine-based sorting signal that is necessary for efficient endocytosis of the P2X(4) receptor. Similar motifs are present in other receptors and may be important for the control of their functional expression.

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Ap2m1  (adaptor related protein complex 2 subunit mu 1)

Additional Information