RGD Reference Report - Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic. - Rat Genome Database

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Chemical-genetic inhibition of a sensitized mutant myosin Vb demonstrates a role in peripheral-pericentriolar membrane traffic.

Authors: Provance, D William  Gourley, Christopher R  Silan, Colleen M  Cameron, L C  Shokat, Kevan M  Goldenring, James R  Shah, Kavita  Gillespie, Peter G  Mercer, John A 
Citation: Provance DW, etal., Proc Natl Acad Sci U S A. 2004 Feb 17;101(7):1868-73. Epub 2004 Feb 6.
RGD ID: 13461756
Pubmed: PMID:14766983   (View Abstract at PubMed)
PMCID: PMC357019   (View Article at PubMed Central)
DOI: DOI:10.1073/pnas.0305895101   (Journal Full-text)

Selective, in situ inhibition of individual unconventional myosins is a powerful approach to determine their specific physiological functions. Here, we report the engineering of a myosin Vb mutant that still hydrolyzes ATP, yet is selectively sensitized to an N(6)-substituted ADP analog that inhibits its activity, causing it to remain tightly bound to actin. Inhibition of the sensitized mutant causes inhibition of accumulation of transferrin in the cytoplasm and increases levels of plasma-membrane transferrin receptor, suggesting that myosin Vb functions in traffic between peripheral and pericentrosomal compartments.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
actin filament binding enablesIMP 13461756PMID:14766983SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Myo5b  (myosin Vb)


Additional Information