RGD Reference Report - Comparison between a-synuclein wild-type and A53T mutation in a progressive Parkinson's disease model. - Rat Genome Database

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Comparison between a-synuclein wild-type and A53T mutation in a progressive Parkinson's disease model.

Authors: Lu, Jianqing  Sun, Feiyi  Ma, Hong  Qing, Hong  Deng, Yulin 
Citation: Lu J, etal., Biochem Biophys Res Commun. 2015 Sep 4;464(4):988-93. doi: 10.1016/j.bbrc.2015.07.007. Epub 2015 Jul 17.
RGD ID: 13452383
Pubmed: PMID:26192120   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2015.07.007   (Journal Full-text)


BACKGROUND AND AIMS: Vector based over-expression of α-synuclein is a newly developed method to establish animal Parkinson's disease (PD) model. In this paper, we inject the rat brain with recombinant adeno-associated virus (rAAV) to express α-synuclein wild-type and A53T mutation, and compared the degeneration of dopaminergic neurons between them.
METHOD AND RESULTS: The rAAV vectors were injected into the substantia nigra pars compacta (SNpc) of rat brain. In different time point, immunohistochemistry was used to detect the expression of α-synuclein. The expression level was lower in the 3rd and 6th week and increased from the 9th week. α-synuclein spread around the neurons in SNpc in the 12th week. The loss of dopaminergic neurons was increasing along the expression of α-synuclein, and damage extent was more serious in the A53T group than the WT group. In the A53T group, there were more insoluble inclusions can be detected, and the phosphorylation of α-synuclein was also higher.
CONLUSION: The result of comparison between the two types of α-synuclein showed that A53T mutated α-synuclein was more effective to establish PD model, and the model based A53T mutated α-synuclein was a suitable model to early-onset PD.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HTTHumanParkinson's disease  IMP  RGD 
HttRatParkinson's disease  ISOHTT (Homo sapiens) RGD 
HttMouseParkinson's disease  ISOHTT (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Htt  (huntingtin)

Genes (Mus musculus)
Htt  (huntingtin)

Genes (Homo sapiens)
HTT  (huntingtin)


Additional Information