RGD Reference Report - Insulin promotes proliferation, survival, and invasion in endometrial carcinoma by activating the MEK/ERK pathway. - Rat Genome Database

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Insulin promotes proliferation, survival, and invasion in endometrial carcinoma by activating the MEK/ERK pathway.

Authors: Wang, Yingmei  Zhu, Yuanxi  Zhang, Lizhi  Tian, Wenyan  Hua, Shaofang  Zhao, Jing  Zhang, Huiying  Xue, Fengxia 
Citation: Wang Y, etal., Cancer Lett. 2012 Sep 28;322(2):223-31. doi: 10.1016/j.canlet.2012.03.026. Epub 2012 Mar 27.
RGD ID: 13441552
Pubmed: PMID:22459351   (View Abstract at PubMed)
DOI: DOI:10.1016/j.canlet.2012.03.026   (Journal Full-text)

The involvement of insulin in endometrial carcinoma (EC) was investigated using radioimmunoassay, Western blot, immunoprecipitation, MTT, and Annexin V-FITC/PI assays in tissue samples and cultured cells. Serum levels of insulin, p-p52Shc, p-p46Shc, Shc·Grb2 complexes, p-MEK, p-ERK, and cyclin D1 were elevated in patients with EC. Expression of key proteins in the MEK/ERK pathway, including p-p52Shc, Shc·Grb2 complexes, p-MEK, p-ERK, and cyclin D1, was significantly higher in patients with advanced FIGO stage, high grade, and lymph-node metastasis and correlated positively with serum insulin concentration. Insulin promotes Ishikawa 3-H-12 cell proliferation, survival, and invasion, and these effects induced by insulin were significantly blocked by MEK inhibitor PD98059. Insulin thus promotes EC cell proliferation, survival, and invasion via the MEK/ERK pathway.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endometrial carcinoma severityIEP 13441552Shc-Grb2 complex and protein:increased expression:serum (human)RGD 
endometrial carcinoma severityISOGRB2 (Homo sapiens)13441552; 13441552Shc-Grb2 complex and protein:increased expression:serum (human)RGD 
endometrial carcinoma severityIEP 13441552; 13441552protein:increased phosphorylation:endometrium (human)RGD 
endometrial carcinoma severityISOMAPK1 (Homo sapiens)13441552; 13441552protein:increased phosphorylation:endometrium (human)RGD 
endometrial carcinoma severityISOMAPK3 (Homo sapiens)13441552; 13441552protein:increased phosphorylation:endometrium (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Grb2  (growth factor receptor bound protein 2)
Mapk1  (mitogen activated protein kinase 1)
Mapk3  (mitogen activated protein kinase 3)

Genes (Mus musculus)
Grb2  (growth factor receptor bound protein 2)
Mapk1  (mitogen-activated protein kinase 1)
Mapk3  (mitogen-activated protein kinase 3)

Genes (Homo sapiens)
GRB2  (growth factor receptor bound protein 2)
MAPK1  (mitogen-activated protein kinase 1)
MAPK3  (mitogen-activated protein kinase 3)


Additional Information