RGD Reference Report - Identification and purification of the carnitine carrier from rat liver mitochondria. - Rat Genome Database

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Identification and purification of the carnitine carrier from rat liver mitochondria.

Authors: Indiveri, C  Tonazzi, A  Palmieri, F 
Citation: Indiveri C, etal., Biochim Biophys Acta. 1990 Oct 24;1020(1):81-6.
RGD ID: 13440120
Pubmed: (View Article at PubMed) PMID:2223786

The carnitine carrier from rat liver mitochondria, solubilized in Triton X-100 and partially purified on hydroxyapatite, was identified and completely purified by specific elution from celite in the presence of cardiolipin. On SDS-gel electrophoresis, the purified celite fraction consisted of a single band with an apparent Mr of 32,500. When reconstituted into liposomes the carnitine transport protein catalyzed an N-ethylmaleimide-sensitive carnitine/carnitine exchange. It was purified 970-fold with a recovery of 43% and a protein yield of 0.04% with respect to the mitochondrial extract. The properties of the reconstituted carrier, i.e., requirement for a countersubstrate, substrate specificity and inhibitor sensitivity, were similar to those of the carnitine transport system as characterized in intact mitochondria.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Slc25a20  (solute carrier family 25 member 20)


Additional Information