RGD Reference Report - Tafazzin knockdown causes hypertrophy of neonatal ventricular myocytes. - Rat Genome Database

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Tafazzin knockdown causes hypertrophy of neonatal ventricular myocytes.

Authors: He, Quan 
Citation: He Q, Am J Physiol Heart Circ Physiol. 2010 Jul;299(1):H210-6. doi: 10.1152/ajpheart.00098.2010. Epub 2010 Mar 26.
RGD ID: 13432160
Pubmed: (View Article at PubMed) PMID:20348225
DOI: Full-text: DOI:10.1152/ajpheart.00098.2010

Mutation of the mitochondrial protein tafazzin causes dilated cardiomyopathy in Barth syndrome. We employed an adenovirus as a vector to transfer tafazzin small hairpin RNA (shRNA) into neonatal ventricular myocytes (NVMs) to investigate the effects of tafazzin knockdown. The tafazzin shRNA adenovirus consistently knocked down tafazzin mRNA and lowered cardiolipin while significantly decreasing the production of ATP by the mitochondria. The phosphorylation of AMP-activated protein kinase and mitochondrial density were both increased in tafazzin knockdown NVMs compared with scrambled shRNA controls. When we tested whether tafazzin knockdown causes hypertrophy in vitro, we found that the surface area of NVMs infected with tafazzin shRNA adenovirus was significantly increased, as were the protein synthesis and expression of the hypertrophic marker gene, brain natriuretic peptide. Taken together, our data support the concept that a decreased tafazzin expression causes cardiomyocyte hypertrophy in vitro.


Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Tafazzin  (tafazzin, phospholipid-lysophospholipid transacylase)

Additional Information