RGD Reference Report - Topoisomerase IIß associates with Ku70 and PARP-1 during double strand break repair of DNA in neurons. - Rat Genome Database

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Topoisomerase IIß associates with Ku70 and PARP-1 during double strand break repair of DNA in neurons.

Authors: Mandraju, Rajakumar  Chekuri, Anil  Bhaskar, C  Duning, Kerstin  Kremerskothen, Joachim  Kondapi, Anand K 
Citation: Mandraju R, etal., Arch Biochem Biophys. 2011 Dec 15;516(2):128-37. doi: 10.1016/j.abb.2011.09.017. Epub 2011 Oct 10.
RGD ID: 13432143
Pubmed: PMID:22019940   (View Abstract at PubMed)
DOI: DOI:10.1016/j.abb.2011.10.001   (Journal Full-text)

In the present study, the activity of Topoisomerase IIß (TopoIIß) is evaluated during peroxide induced double stranded DNA breaks (DSBs) repair in primary neurons. The results showed that the TopoIIß levels were enhanced during recovery from peroxide mediated damage (PED) along with Ku70, PARP-1, pol beta, and WRN helicase. Furthermore, siRNA mediated knock-down of TopoIIß in primary neurons conferred enhanced susceptibility to PED in neurons. DSBs in neurons are repaired through two pathways, one promoted by Ku70, while the other is by PARP-1 dependent manner. Participation of TopoIIß in both pathways was assessed by analysis of the interaction of TopoIIß with Ku70 and PARP-1 using co-immunoprecipitation experiments in extracts of neurons under peroxide treatment and recovery. The results of these studies showed a strong interaction of TopoIIß with Ku70 as well as PARP-1 suggesting that TopoIIß is associated both in Ku70 and PARP-dependent pathways in DSBs repair in primary neurons. The study has thus established that TopoIIß is an essential component in DSBs repair in primary neurons in both Ku70 and PARP-1 dependent pathways. We suppose that the interaction of TopoIIß may provide stabilization of the repair complex, which may assist in maintenance of tensional integrity in genomic DNA.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Top2b  (DNA topoisomerase II beta)


Additional Information