RGD Reference Report - Expression of rat kidney anion exchanger 1 in type A intercalated cells in metabolic acidosis and alkalosis. - Rat Genome Database

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Expression of rat kidney anion exchanger 1 in type A intercalated cells in metabolic acidosis and alkalosis.

Authors: Huber, S  Asan, E  Jöns, T  Kerscher, C  Püschel, B  Drenckhahn, D 
Citation: Huber S, etal., Am J Physiol. 1999 Dec;277(6 Pt 2):F841-9.
RGD ID: 13208945
Pubmed: (View Article at PubMed) PMID:10600930

By enzyme-linked in situ hybridization (ISH), direct evidence is provided that acid-secreting intercalated cells (type A IC) of both the cortical and medullary collecting ducts of the rat kidney selectively express the mRNA of the kidney splice variant of anion exchanger 1 (kAE1) and no detectable levels of the erythrocyte AE1 (eAE1) mRNA. Using single-cell quantification by microphotometry of ISH enzyme reaction, medullary type A IC were found to contain twofold higher kAE1 mRNA levels compared with cortical type A IC. These differences correspond to the higher intensity of immunostaining in medullary versus cortical type A IC. Chronic changes of acid-base status induced by addition of NH(4)Cl (acidosis) or NaHCO3 (alkalosis) to the drinking water resulted in up to 35% changes of kAE1 mRNA levels in both cortical and medullary type A IC. These experiments provide direct evidence at the cellular level of kAE1 expression in type A IC and show moderate capacity of type A IC to respond to changes of acid-base status by modulation of kAE1 mRNA levels.



Disease Annotations    
Alkalosis  (IEP,ISO)
renal tubular acidosis  (IEP,ISO)

Gene Ontology Annotations    

Biological Process

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Slc4a1  (solute carrier family 4 member 1 (Diego blood group))

Genes (Mus musculus)
Slc4a1  (solute carrier family 4 (anion exchanger), member 1)

Genes (Homo sapiens)
SLC4A1  (solute carrier family 4 member 1 (Diego blood group))


Additional Information