RGD Reference Report - Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys. - Rat Genome Database

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Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys.

Authors: Saito, Akira  Miyauchi, Naoko  Hashimoto, Taeko  Karasawa, Tamaki  Han, Gi Dong  Kayaba, Mutsumi  Sumi, Tomoyuki  Tomita, Masayuki  Ikezumi, Yohei  Suzuki, Kenji  Koitabashi, Yasushi  Shimizu, Fujio  Kawachi, Hiroshi 
Citation: Saito A, etal., Am J Physiol Regul Integr Comp Physiol. 2011 Feb;300(2):R340-8. doi: 10.1152/ajpregu.00640.2009. Epub 2010 Nov 3.
RGD ID: 13207335
Pubmed: PMID:21048075   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpregu.00640.2009   (Journal Full-text)

The slit diaphragm connecting the adjacent foot processes of glomerular epithelial cells (podocytes) is the final barrier of the glomerular capillary wall and serves to prevent proteinuria. Podocytes are understood to be terminally differentiated cells and share some common features with neurons. Neurexin is a presynaptic adhesion molecule that plays a role in synaptic differentiation. Although neurexin has been understood to be specifically expressed in neuronal tissues, we found that neurexin was expressed in several organs. Several forms of splice variants of neurexin-1α were detected in the cerebrum, but only one form of neurexin-1α was detected in glomeruli. Immunohistochemical study showed that neurexin restrictedly expressed in the podocytes in kidneys. Dual-labeling analyses showed that neurexin was colocalized with CD2AP, an intracellular component of the slit diaphragm. Immunoprecipitation assay using glomerular lysate showed that neurexin interacted with CD2AP and CASK. These observations indicated that neurexin localized at the slit diaphragm area. The staining intensity of neurexin in podocytes was clearly lowered, and their staining pattern shifted to a more discontinuous patchy pattern in the disease models showing severe proteinuria. The expression and localization of neurexin in these models altered more clearly and rapidly than that of other slit diaphragm components. We propose that neurexin is available as an early diagnostic marker to detect podocyte injury. Neurexin coincided with nephrin, a key molecule of the slit diaphragm detected in a presumptive podocyte of the developing glomeruli and in the glomeruli for which the slit diaphragm is repairing injury. These observations suggest that neurexin is involved in the formation of the slit diaphragm and the maintenance of its function.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nrxn1Ratslit diaphragm located_inIDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nrxn1  (neurexin 1)

Additional Information