RGD Reference Report - Protective effect of naringenin in experimental ischemic stroke: down-regulated NOD2, RIP2, NF-¿B, MMP-9 and up-regulated claudin-5 expression. - Rat Genome Database
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) RatMine GViewer (Genome Viewer) Overgo Probe Designer ACP Haplotyper Genome Scanner
Aging & Age-Related Disease Cancer Cardiovascular Disease COVID-19 Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models PhenoMiner (Quantitative Phenotypes) Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes GERRC (Gene Editing Rat Resource Center) Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Poster Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Protective effect of naringenin in experimental ischemic stroke: down-regulated NOD2, RIP2, NF-¿B, MMP-9 and up-regulated claudin-5 expression.

Authors: Bai, Xue  Zhang, Xiangjian  Chen, Linyu  Zhang, Jian  Zhang, Lan  Zhao, Xumeng  Zhao, Ting  Zhao, Yuan 
Citation: Bai X, etal., Neurochem Res. 2014 Aug;39(8):1405-15. doi: 10.1007/s11064-014-1326-y. Epub 2014 May 20.
RGD ID: 13204729
Pubmed: (View Article at PubMed) PMID:24842554
DOI: Full-text: DOI:10.1007/s11064-014-1326-y

Inflammatory damage plays a pivotal, mainly detrimental role in cerebral ischemic pathogenesis and may represent a promising target for treatment. Naringenin (NG) has gained growing appreciation for its beneficial biological effects through its anti-inflammatory property. Whether this protective effect applies to cerebral ischemic injury, we therefore investigate the potential neuroprotective role of NG and the underlying mechanisms. Focal cerebral ischemia in male Sprague-Dawley rats was induced by permanent middle cerebral artery occlusion (pMCAO) and NG was pre-administered intragastrically once daily for four consecutive days before surgery. Neurological deficit, brain water content and infarct volume were measured at 24 h after stroke. Immunohistochemistry, Western blot and RT-qPCR were used to explore the anti-inflammatory potential of NG in the regulation of NOD2, RIP2 and NF-κB in ischemic cerebral cortex. Additionally, the activities of MMP-9 and claudin-5 were analyzed to detect NG's influence on blood-brain barrier. Compared with pMCAO and Vehicle groups, NG noticeably improved neurological deficit, decreased infarct volume and edema at 24 h after ischemic insult. Consistent with these results, our data also indicated that NG significantly downregulated the expression of NOD2, RIP2, NF-κB and MMP-9, and upregulated the expression of claudin-5 (P < 0.05). The results provided a neuroprotective profile of NG in cerebral ischemia, this effect was likely exerted by down-regulated NOD2, RIP2, NF-κB, MMP-9 and up-regulated claudin-5 expression.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Cldn5  (claudin 5)
Mmp9  (matrix metallopeptidase 9)
Nod2  (nucleotide-binding oligomerization domain containing 2)

Genes (Mus musculus)
Cldn5  (claudin 5)
Mmp9  (matrix metallopeptidase 9)
Nod2  (nucleotide-binding oligomerization domain containing 2)

Genes (Homo sapiens)
CLDN5  (claudin 5)
MMP9  (matrix metallopeptidase 9)
NOD2  (nucleotide binding oligomerization domain containing 2)


Additional Information