RGD Reference Report - Kinesin 3 and cytoplasmic dynein mediate interkinetic nuclear migration in neural stem cells. - Rat Genome Database

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Kinesin 3 and cytoplasmic dynein mediate interkinetic nuclear migration in neural stem cells.

Authors: Tsai, Jin-Wu  Lian, Wei-Nan  Kemal, Shahrnaz  Kriegstein, Arnold R  Vallee, Richard B 
Citation: Tsai JW, etal., Nat Neurosci. 2010 Dec;13(12):1463-71. doi: 10.1038/nn.2665. Epub 2010 Oct 31.
RGD ID: 13204706
Pubmed: PMID:21037580   (View Abstract at PubMed)
PMCID: PMC3059207   (View Article at PubMed Central)
DOI: DOI:10.1038/nn.2665   (Journal Full-text)

Radial glial progenitor cells exhibit bidirectional cell cycle-dependent nuclear oscillations. The purpose and underlying mechanism of this unusual 'interkinetic nuclear migration' are poorly understood. We investigated the basis for this behavior by live imaging of nuclei, centrosomes and microtubules in embryonic rat brain slices, coupled with the use of RNA interference (RNAi) and the myosin inhibitor blebbistatin. We found that nuclei migrated independent of centrosomes and unidirectionally away from or toward the ventricular surface along microtubules, which were uniformly oriented from the ventricular surface to the pial surface of the brain. RNAi directed against cytoplasmic dynein specifically inhibited nuclear movement toward the apical surface. An RNAi screen of kinesin genes identified Kif1a, a member of the kinesin-3 family, as the motor for basally directed nuclear movement. These observations provide direct evidence that kinesins are involved in nuclear migration and neurogenesis and suggest that a cell cycle-dependent switch between distinct microtubule motors drives interkinetic nuclear migration.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Kif1aRatinterkinetic nuclear migration  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kif1a  (kinesin family member 1A)


Additional Information