RGD Reference Report - RNAi reveals doublecortin is required for radial migration in rat neocortex. - Rat Genome Database

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RNAi reveals doublecortin is required for radial migration in rat neocortex.

Authors: Bai, J  Ramos, RL  Ackman, JB  Thomas, AM  Lee, RV  LoTurco, JJ 
Citation: Bai J, etal., Nat Neurosci 2003 Dec;6(12):1277-83. Epub 2003 Nov 16.
RGD ID: 1304461
Pubmed: PMID:14625554   (View Abstract at PubMed)
DOI: DOI:10.1038/nn1153   (Journal Full-text)

Mutations in the doublecortin gene (DCX) in humans cause malformation of the cerebral neocortex. Paradoxically, genetic deletion of Dcx in mice does not cause neocortical malformation. We used electroporation of plasmids encoding short hairpin RNA to create interference (RNAi) of DCX protein in utero, and we show that DCX is required for radial migration in developing rat neocortex. RNAi of DCX causes both cell-autonomous and non-cell autonomous disruptions in radial migration, and creates two disruptions in neocortical development. First, many neurons prematurely stop migrating to form subcortical band heterotopias within the intermediate zone and then white matter. Second, many neurons migrate into inappropriate neocortical lamina within normotopic cortex. In utero RNAi can therefore be effectively used to study the specific cellular roles of DCX in neocortical development and to produce an animal model of double cortex syndrome.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
DcxRatneuron migration  IMP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
DcxRatabnormal brain development  IMP  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Dcx  (doublecortin)


Additional Information