RGD Reference Report - Phospholipase Cdelta(1) does not mediate Ca(2+) responses in neonatal rat cardiomyocytes. - Rat Genome Database

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Phospholipase Cdelta(1) does not mediate Ca(2+) responses in neonatal rat cardiomyocytes.

Authors: Woodcock, EA  Mitchell, CJ  Biden, TJ 
Citation: Woodcock EA, etal., FEBS Lett 2003 Jul 10;546(2-3):325-8.
RGD ID: 1304396
Pubmed: PMID:12832062   (View Abstract at PubMed)

Phospholipase C (PLC) activation in neonatal rat ventricular cardiomyocytes (NRVM) generates inositol(1,4,5)trisphosphate (Ins(1,4,5)P(3)) in response to elevations in Ca(2+) or inositol(1,4)bisphosphate in response to G protein stimulation. Overexpression of PLCdelta(1) increased total [(3)H]inositol phosphate (InsP) content and elevated [(3)H]Ins(1,4,5)P(3), but failed to increase [(3)H]InsP responses to the Ca(2+) ionophore A23187. Antisense PLCdelta(1) expression reduced endogenous PLCdelta(1) content but did not decrease the A23187 response. In permeabilized NRVM, [(3)H]InsP responses to elevated Ca(2+) were not inhibited by Ins(1,4,5)P(3), even at concentrations 1000-fold greater than required for selective inhibition of PLCdelta(1). Taken together these data provide evidence that PLCdelta(1) does not mediate the InsP response to elevated Ca(2+) in NRVM.

Objects referenced in this article
Gene Plcd1 phospholipase C, delta 1 Rattus norvegicus

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