RGD Reference Report - The orphan nuclear receptor NOR-1 interacts with the homeobox containing protein Six3. - Rat Genome Database

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The orphan nuclear receptor NOR-1 interacts with the homeobox containing protein Six3.

Authors: Ohkura, N  Ohkubo, T  Maruyama, K  Tsukada, T  Yamaguchi, K 
Citation: Ohkura N, etal., Dev Neurosci 2001;23(1):17-24.
RGD ID: 1304226
Pubmed: PMID:11173923   (View Abstract at PubMed)
DOI: DOI:48692   (Journal Full-text)

Neuron-derived orphan receptor 1 (NOR-1) is a member of the NGFI-B subfamily within the nuclear receptor superfamily. In order to identify cofactors that associate with NOR-1 in the fetal forebrain, we tested a yeast two-hybrid system with the NOR-1 cDNA fragment lacking a transactivating domain as a bait. By screening of the rat fetal brain embryonic day 17 library, a rat homologue of Six3 was identified as an associated protein. We demonstrated that NOR-1 interacted with Six3 in yeast and in vitro, and the association was required for the DNA binding and AF2 domains of NOR-1. Regarding the other members of the family (NGFI-B and RNR-1), association with Six3 was not observed in yeast. In addition, cotransfection experiments with Six3 and NOR-1 indicated that Six3 had a negative activity against the transactivation by NOR-1 through the NBRE response element in a dose-dependent manner. The overlap in expression of NOR-1 and Six3 was mainly detected in the rat fetal forebrain on embryonic day 18. Thereafter, the expression of both genes diminished rapidly. These results suggest that a dimer consisting of a homeobox containing protein Six3 and transcriptional factor NOR-1 might regulate gene expression during the late stage of the fetal forebrain development. This study provides, after the association of Ftz and Ftz-F1 in Drosophila, another example of a dimer formation of a homeobox protein and an orphan nuclear receptor.

Objects referenced in this article
Gene Six3 SIX homeobox 3 Rattus norvegicus

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