RGD Reference Report - Cloning of rat ABCA7 and its preferential expression in platelets. - Rat Genome Database

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Cloning of rat ABCA7 and its preferential expression in platelets.

Authors: Sasaki, M  Shoji, A  Kubo, Y  Nada, S  Yamaguchi, A 
Citation: Sasaki M, etal., Biochem Biophys Res Commun 2003 May 16;304(4):777-82.
RGD ID: 1303958
Pubmed: PMID:12727224   (View Abstract at PubMed)

We cloned the full-length cDNA of a rat orthologue of ABCA7 (rABCA7) from rat platelets. The cDNA of rABCA7 is 6510bp in length and encodes a protein of 2170 amino acids. The amino acid sequence of rABCA7 exhibits homology to those of mouse ABCA7 (92.5% identical in amino acid sequence) and human ABCA7 (76.6%). We obtained two clones of monoclonal antibodies against rABCA7 recognizing different epitopes. Analysis of CHO cells stably expressing rABCA7 by confocal laser-scanning microscopy indicated that rABCA7 is mainly located in the plasma membrane. Western blot analysis of rat tissues revealed that rABCA7 was preferentially expressed in platelets and that its apparent molecular mass was 250kDa. This is the first report of the tissue distribution of rABCA7 at the protein level and is the first reported case of ABC transporters being expressed in platelets, suggesting their important role in platelet function.

Objects referenced in this article
Gene Abca7 ATP binding cassette subfamily A member 7 Rattus norvegicus

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