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Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat.

Authors: Michalkiewicz, M  Michalkiewicz, T  Ettinger, RA  Rutledge, EA  Fuller, JM  Moralejo, DH  Van Yserloo, B  MacMurray, AJ  Kwitek, AE  Jacob, HJ  Lander, ES  Lernmark, A 
Citation: Michalkiewicz M, etal., Physiol Genomics 2004 Oct 4;19(2):228-32. Epub 2004 Aug 24.
Pubmed: (View Article at PubMed) PMID:15328390
DOI: Full-text: DOI:10.1152/physiolgenomics.00126.2004

A single point mutation in a novel immune-associated nucleotide gene 5 (Ian5) coincides with severe T cell lymphopenia in BB rats. We used a transgenic rescue approach in lymphopenic BB-derived congenic F344.lyp/lyp rats to determine whether this mutation is responsible for lymphopenia and to establish the functional importance of this novel gene. A 150-kb P1 artificial chromosome (PAC) transgene harboring a wild-type allele of the rat Ian5 gene restored Ian5 transcript and protein levels, completely rescuing the T cell lymphopenia in the F344.lyp/lyp rats. This successful complementation provides direct functional evidence that the Ian5 gene product is essential for maintaining normal T cell levels. It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene.


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RGD Object Information
RGD ID: 1303374
Created: 2004-12-09
Species: All species
Last Modified: 2004-12-09
Status: ACTIVE


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