RGD Reference Report - Rim, a component of the presynaptic active zone and modulator of exocytosis, binds 14-3-3 through its N terminus. - Rat Genome Database

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Rim, a component of the presynaptic active zone and modulator of exocytosis, binds 14-3-3 through its N terminus.

Authors: Sun, L  Bittner, MA  Holz, RW 
Citation: Sun L, etal., J Biol Chem 2003 Oct 3;278(40):38301-9. Epub 2003 Jul 18.
RGD ID: 1302925
Pubmed: PMID:12871946   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M212801200   (Journal Full-text)

Rim1, a brain-specific Rab3a-binding protein, localizes to the presynaptic cytomatrix and plays an important role in synaptic transmission and synaptic plasticity. Rim2, a homologous protein, is more ubiquitously expressed and is found in neuroendocrine cells as well as in brain. Both Rim1 and Rim2 contain multiple domains, including an N-terminal zinc finger, which in Rim1 strongly enhances secretion in chromaffin and PC12 cells. The yeast two-hybrid technique identified 14-3-3 proteins as ligands of the N-terminal domain. In vitro protein binding experiments confirmed a high-affinity interaction between the N terminus of Rim1 and 14-3-3. The N-terminal domain of Rim2 also bound 14-3-3. The binding domains were localized to a short segment just C-terminal to the zinc finger. 14-3-3 proteins bind to specific phosphoserine residues. Alkaline phosphatase treatment of N-terminal domains of Rim1 and Rim2 almost completely inhibited the binding of 14-3-3. Two serine residues in Rim1 (Ser-241 and Ser-287) and one serine residue in Rim2 (Ser-335) were required for 14-3-3 binding. Incubation with Ca2+/calmodulin-dependent protein kinase II greatly stimulated the interaction of recombinant N-terminal Rim but not the S241/287A mutant with 14-3-3, again indicating the importance of the phosphorylation of these residues for the binding. Rabphilin3, another Rab3a effector, also bound 14-3-3. Serine-to-alanine mutations identified Ser-274 as the likely phosphorylated residue to which 14-3-3 binds. Because the phosphorylation of this residue had been shown to be stimulated upon depolarization in brain slices, the interaction of 14-3-3 with Rabphilin3 may be important in the dynamic function of central nervous system neurons.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
exocytosis  TAS 1302925 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rims2  (regulating synaptic membrane exocytosis 2)

Objects referenced in this article
Gene Rims1 regulating synaptic membrane exocytosis 1 Rattus norvegicus
Gene Ywhah tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta Rattus norvegicus

Additional Information