RGD Reference Report - Identification and characterization of a novel Rho GTPase activating protein implicated in receptor-mediated endocytosis. - Rat Genome Database

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Identification and characterization of a novel Rho GTPase activating protein implicated in receptor-mediated endocytosis.

Authors: Sakakibara, T  Nemoto, Y  Nukiwa, T  Takeshima, H 
Citation: Sakakibara T, etal., FEBS Lett 2004 May 21;566(1-3):294-300.
RGD ID: 1302833
Pubmed: PMID:15147912   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2004.03.101   (Journal Full-text)

Cbl-interacting protein of 85 kDa (CIN85) is a recently identified adaptor protein involved in the endocytic process of several receptor tyrosine kinases. Here we have identified a novel RhoGAP, CIN85 associated multi-domain containing Rho1 (CAMGAP1) as a binding protein for CIN85. CAMGAP1 is composed of an Src homology 3 (SH3) domain, multiple WW domains, a proline-rich region, a PH domain and a RhoGAP domain, and has the domain architecture similar to ARHGAP9 and ARHGAP12. CAMGAP1 mRNA is widely distributed in murine tissues. Biochemical assays showed its GAP activity toward Rac1 and Cdc42. Protein binding and expression studies indicated that the second SH3 domain of CIN85 binds to a proline-rich region of CAMGAP1. Overexpression of a truncated form of CAMGAP1 interferes with the internalization of transferrin receptors, suggesting that CAMGAP1 may play a role in clathrin-mediated endocytosis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
receptor-mediated endocytosis  IMP 1302833 RGD 
receptor-mediated endocytosis involved_inIMP 1302833PMID:15147912HGNC-UCL 
receptor-mediated endocytosis acts_upstream_of_or_withinIDA 1302833PMID:15147912MGI 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
endosome located_inIDA 1302833PMID:15147912MGI 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Arhgap27  (Rho GTPase activating protein 27)
Sh3kbp1  (SH3 domain-containing kinase-binding protein 1)

Objects referenced in this article
Gene ARHGAP27 Rho GTPase activating protein 27 Homo sapiens

Additional Information