RGD Reference Report - Functional and genetic analysis of two CD8 T cell subsets defined by the level of CD45RC expression in the rat. - Rat Genome Database

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Functional and genetic analysis of two CD8 T cell subsets defined by the level of CD45RC expression in the rat.

Authors: Xystrakis, E  Cavailles, P  Dejean, AS  Cautain, B  Colacios, C  Lagrange, D  Van de Gaar, MJ  Bernard, I  Gonzalez-Dunia, D  Damoiseaux, J  Fournie, GJ  Saoudi, A 
Citation: Xystrakis E, etal., J Immunol 2004 Sep 1;173(5):3140-7.
RGD ID: 1302824
Pubmed: (View Article at PubMed) PMID:15322174

Differential cytokine production by T cells plays an important role in the outcome of the immune response. We show that the level of CD45RC expression differentiates rat CD8 T cells in two subpopulations, CD45RC(high) and CD45RC(low), that have different cytokine profiles and functions. Upon in vitro stimulation, in an Ag-presenting cell-independent system, CD45RC(high) CD8 T cells produce IL-2 and IFN-gamma while CD45RC(low) CD8 T cells produce IL-4, IL-10, and IL-13. In vitro, these subsets also exhibit different cytotoxic and suppressive functions. The CD45RC(high)/CD45RC(low) CD8 T cell ratio was determined in Lewis (LEW) and Brown-Norway (BN) rats. These two rat strains differ with respect to the Th1/Th2 polarization of their immune responses and to their susceptibility to develop distinct immune diseases. The CD45RC(high)/CD45RC(low) CD8 T cell ratio is higher in LEW than in BN rats, and this difference is dependent on hemopoietic cells. Linkage analysis in a F(2)(LEW x BN) intercross identified two quantitative trait loci on chromosomes 9 and 20 controlling the CD45RC(high)/CD45RC(low) CD8 T cell ratio. This genetic control was confirmed in congenic rats. The region on chromosome 9 was narrowed down to a 1.2-cM interval that was found to also control the IgE response in a model of Th2-mediated disorder. Identification of genes that control the CD45RC(high)/CD45RC(low) CD8 T cell subsets in these regions could be of great interest for the understanding of the pathophysiology of immune-mediated diseases.



Disease Annotations    

Phenotype Annotations    

Mammalian Phenotype

Objects Annotated

QTLs
Cdexp1  (CD45RC expression in CD8 T cells QTL 1)
Cdexp2  (CD45RC expression in CD8 T cells QTL 2)


Additional Information