RGD Reference Report - Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures. - Rat Genome Database

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Mice lacking the 65 kDa isoform of glutamic acid decarboxylase (GAD65) maintain normal levels of GAD67 and GABA in their brains but are susceptible to seizures.

Authors: Asada, H  Kawamura, Y  Maruyama, K  Kume, H  Ding, R  Ji, FY  Kanbara, N  Kuzume, H  Sanbo, M  Yagi, T  Obata, K 
Citation: Asada H, etal., Biochem Biophys Res Commun 1996 Dec 24;229(3):891-5.
RGD ID: 1302511
Pubmed: PMID:8954991   (View Abstract at PubMed)

The gene encoding of the 65 kDa isoform of the gamma-aminobutyric acid (GABA)-synthesizing enzyme, glutamic acid decarboxylase (GAD), GAD65, was targeted in mice by homologous recombination. Viable GAD65 -/- mice were obtained with the expected mendelian frequency and displayed no gross morphological defects. Despite the complete loss of GAD65 mRNA and protein in a homozygous mutant, there was no difference in GABA content in the brains of GAD65 +/+, +/-, and -/- mice. As for the other 67 kDa isoform (GAD67), the levels of mRNA and protein were largely unchanged by the GAD65 mutation. General behavior, including locomotor activity and performance in the Morris water maze task, appeared normal, but seizures were more easily induced by picrotoxin and pentylenetetrazol: the latencies to seizures induced by picrotoxin were shorter and the dose of pentylenetetrazol required for induction of seizures was lower.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
visual epilepsy susceptibilityISOGad2 (Mus musculus)1302511; 1302511 RGD 
visual epilepsy susceptibilityIMP 1302511 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gad2  (glutamate decarboxylase 2)

Genes (Mus musculus)
Gad2  (glutamic acid decarboxylase 2)

Genes (Homo sapiens)
GAD2  (glutamate decarboxylase 2)

Objects referenced in this article
Gene Gad1 glutamate decarboxylase 1 Mus musculus

Additional Information