RGD Reference Report - CLIC6, a member of the intracellular chloride channel family, interacts with dopamine D(2)-like receptors. - Rat Genome Database

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CLIC6, a member of the intracellular chloride channel family, interacts with dopamine D(2)-like receptors.

Authors: Griffon, N  Jeanneteau, F  Prieur, F  Diaz, J  Sokoloff, P 
Citation: Griffon N, etal., Brain Res Mol Brain Res 2003 Sep 10;117(1):47-57.
RGD ID: 1302428
Pubmed: PMID:14499480   (View Abstract at PubMed)

To identify proteins interacting with the C-terminal cytoplasmic tail of the dopamine D(3) receptor (D(3)R), we used the two-hybrid system to screen a rat brain cDNA library. We isolated three partial cDNAs encoding, respectively, for the MUPP1 multi-PDZ protein, for the N-terminal region of radixin, for GIPC and for a 160-amino acid open reading frame sharing high homology with the human CLIC6, also identified as parchorin in rabbit. In the two-hybrid system, CLIC6 was also able to interact with the D(2)R and D(4)R. The interaction between D(3)R and CLIC6 was confirmed by the use of a GST-D(3)R C-terminus fusion protein and COS cell extracts transiently expressing epitope-tagged CLIC6. In adult rat brain, CLIC6 mRNA expression was restricted to the choroid plexus, the striatal proliferative subventricular zone and the cerebellum where it is co-expressed with the D(3)R in the Purkinje cells of the lobules IX and X. CLIC6 mRNA was also detected in the pituitary in the posterior lobe and in cells co-expressing the D(2)R at the border between the intermediate and anterior lobes. In transfected HEK293 cells, D(3)R and CLIC6 co-localized at the plasma-membrane. No effect of CLIC6 transfection was observed on either intracellular chloride concentration or D(3)R/D(2)R-mediated response. In two-hybrid system, CLIC6 also interacted with MUPP1 and radixin but not GIPC, suggesting it could take part in a complex with D(2)-like receptors, not only by direct interaction with their C-termini, but also through interactions with scaffolding proteins.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Clic6  (chloride intracellular channel 6)
Drd2  (dopamine receptor D2)
Drd3  (dopamine receptor D3)
Drd4  (dopamine receptor D4)

Objects referenced in this article
Gene Clic6 chloride intracellular channel 6 Mus musculus
Gene Mpdz multiple PDZ domain crumbs cell polarity complex component Rattus norvegicus
Gene Rdx radixin Rattus norvegicus

Additional Information