RGD Reference Report - Characterization of ovarian carbonyl reductase gene expression during ovulation in the gonadotropin-primed immature Rat. - Rat Genome Database

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Characterization of ovarian carbonyl reductase gene expression during ovulation in the gonadotropin-primed immature Rat.

Authors: Espey, LL  Yoshioka, S  Russell, D  Ujioka, T  Vladu, B  Skelsey, M  Fujii, S  Okamura, H  Richards, JS 
Citation: Espey LL, etal., Biol Reprod 2000 Feb;62(2):390-7.
RGD ID: 1299856
Pubmed: PMID:10642578   (View Abstract at PubMed)

In this differential-display polymerase chain reaction-based study, four different primer sets generated cDNA fragments of ovarian carbonyl reductase genes that were uniquely expressed during the ovulatory process in eCG-primed immature rats. The temporal pattern of expression of this aldo-keto reductase gene was delineated by extracting ovarian RNA at 0, 2, 4, 8, 12, and 24 h after induction of ovulation via injection of the primed animals with hCG. The results showed that at least four homologous forms of this gene were transcribed during ovulation. Northern blot analyses indicated a 14-fold increase in ovarian mRNA for carbonyl reductase, with expression reaching a peak at 8 h after hCG treatment and then declining to negligible levels during the next 16 h. In situ hybridization revealed that most of the transcription was in the thecal connective tissue of the ovary and was absent from the granulosa layer of ovarian follicles. Treatment of the animals with ovulation-blocking doses of epostane (an inhibitor of progesterone synthesis) or indomethacin (an inhibitor of prostanoid synthesis) did not reduce the expression of ovarian carbonyl reductase. Nevertheless, the temporal pattern of expression of carbonyl reductase after the induction of ovulation suggests that this enzyme activity is at least indirectly associated with the ovulatory process.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ovulation from ovarian follicle  IEP 1299856 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cbr1  (carbonyl reductase 1)


Additional Information