RGD Reference Report - Identification of neuropeptide W as the endogenous ligand for orphan G-protein-coupled receptors GPR7 and GPR8. - Rat Genome Database

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Identification of neuropeptide W as the endogenous ligand for orphan G-protein-coupled receptors GPR7 and GPR8.

Authors: Shimomura, Y  Harada, M  Goto, M  Sugo, T  Matsumoto, Y  Abe, M  Watanabe, T  Asami, T  Kitada, C  Mori, M  Onda, H  Fujino, M 
Citation: Shimomura Y, etal., J Biol Chem 2002 Sep 27;277(39):35826-32. Epub 2002 Jul 18.
RGD ID: 1299572
Pubmed: PMID:12130646   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M205337200   (Journal Full-text)

The structurally related orphan G-protein-coupled receptors GPR7 and GPR8 are expressed in the central nervous system, and their ligands have not been identified. Here, we report the identification of the endogenous ligand for both of these receptors. We purified the peptide ligand from porcine hypothalamus using stable Chinese hamster ovary cell lines expressing human GPR8 and cloned the cDNA encoding its precursor protein. The cDNA encodes two forms of the peptide ligand with lengths of 23 and 30 amino acid residues as mature peptides. We designated the two ligands neuropeptide W-23 (NPW23) and neuropeptide W-30 (NPW30). The amino acid sequence of NPW23 is completely identical to that of the N-terminal 23 residues of NPW30. Synthetic NPW23 and NPW30 activated and bound to both GPR7 and GPR8 at similar effective doses. Intracerebroventricular administration of NPW23 in rats increased food intake and stimulated prolactin release. These findings indicate that neuropeptide W is the endogenous ligand for both GPR7 and GPR8 and acts as a mediator of the central control of feeding and the neuroendocrine system.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
G protein-coupled receptor signaling pathway  IMP 1299572 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
G protein-coupled receptor binding  IMP 1299572 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Npw  (neuropeptide W)


Additional Information