RGD Reference Report - Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56. - Rat Genome Database

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Cloning of rat sp56, the homologue of mouse sperm ZP3 receptor-sp56.

Authors: He, XB  Yan, YC  Li, YP  Koide, SS 
Citation: He XB, etal., Cell Res 2003 Apr;13(2):121-9.
RGD ID: 1299568
Pubmed: PMID:12737520   (View Abstract at PubMed)
DOI: DOI:10.1038/sj.cr.7290156   (Journal Full-text)

Mouse sp56 is considered as one of the candidates for mouse zona pellucida 3 (mZP3) receptor. Up to date, its homologue has only been cloned from guinea pig, namely AM67. Based on the cDNA sequence of mouse sp56, we designed a pair of primer to amplify its homologue from rat testis cDNA. Using RT-PCR, two fragments of 743 bp and 938 bp were amplified. The PCR products show very high homology to mouse sp56. However, the 743 bp product completely lacks one of the seven Sushi domains of mouse sp56. Using the 743 bp product as the probe to detect the expression profile of sp56 in rat tissues, Northern blot shows that a approximately 2.0 kb mRNA expresses specifically in testis. Employed the RACE method, two full cDNA sequences of rat sp56 were obtained. A Mr approximately 42 KD band was detected in denatured and non-reducing protein sample of rat testis and sperm with anti-mouse sp56 monoclonal antibody by Western blot method. Rat sp56 was localized on rat sperm head by the indirect immunofluorescence method. Rat sp56 immunoreactivity was detected from the early pachytene spermatocytes and throughout the spermatogenesis. Its cloning will further our understanding of the mechanism of the sperm-egg recognition and binding.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Zp3rRatbinding of sperm to zona pellucida  IMP  RGD 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Zp3rRatacrosomal matrix located_inIDA PMID:12737520UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Zp3r  (zona pellucida 3 receptor)

Additional Information