RGD Reference Report - Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs. - Rat Genome Database

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Gene discovery in the hamster: a comparative genomics approach for gene annotation by sequencing of hamster testis cDNAs.

Authors: Oduru, S  Campbell, JL  Karri, S  Hendry, WJ  Khan, SA  Williams, SC 
Citation: Oduru S, etal., BMC Genomics 2003 Jun 3;4(1):22.
RGD ID: 1299549
Pubmed: PMID:12783626   (View Abstract at PubMed)
PMCID: PMC161800   (View Article at PubMed Central)
DOI: DOI:10.1186/1471-2164-4-22   (Journal Full-text)

BACKGROUND: Complete genome annotation will likely be achieved through a combination of computer-based analysis of available genome sequences combined with direct experimental characterization of expressed regions of individual genomes. We have utilized a comparative genomics approach involving the sequencing of randomly selected hamster testis cDNAs to begin to identify genes not previously annotated on the human, mouse, rat and Fugu (pufferfish) genomes. RESULTS: 735 distinct sequences were analyzed for their relatedness to known sequences in public databases. Eight of these sequences were derived from previously unidentified genes and expression of these genes in testis was confirmed by Northern blotting. The genomic locations of each sequence were mapped in human, mouse, rat and pufferfish, where applicable, and the structure of their cognate genes was derived using computer-based predictions, genomic comparisons and analysis of uncharacterized cDNA sequences from human and macaque. CONCLUSION: The use of a comparative genomics approach resulted in the identification of eight cDNAs that correspond to previously uncharacterized genes in the human genome. The proteins encoded by these genes included a new member of the kinesin superfamily, a SET/MYND-domain protein, and six proteins for which no specific function could be predicted. Each gene was expressed primarily in testis, suggesting that they may play roles in the development and/or function of testicular cells.

Objects referenced in this article
Gene CCDC186 coiled-coil domain containing 186 Homo sapiens
Gene Kif27 kinesin family member 27 Rattus norvegicus

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