RGD Reference Report - Growth-related renal type II Na/Pi cotransporter. - Rat Genome Database

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Growth-related renal type II Na/Pi cotransporter.

Authors: Segawa, H  Kaneko, I  Takahashi, A  Kuwahata, M  Ito, M  Ohkido, I  Tatsumi, S  Miyamoto, K 
Citation: Segawa H, etal., J Biol Chem 2002 May 31;277(22):19665-72. Epub 2002 Mar 5.
RGD ID: 1299524
Pubmed: PMID:11880379   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M200943200   (Journal Full-text)

Growth is critically dependent on the retention of a variety of nutrients. The kidney contributes to this positive external balance. In the present study, we isolated a cDNA from the human and rat kidney that encodes a growth-related Na(+)-dependent inorganic phosphate (P(i)) cotransporter (type IIc). Microinjection of type IIc cRNA into Xenopus oocytes demonstrated sodium-dependent P(i) cotransport activity. Affinity for P(i) was 0.07 mm in 100 mm Na(+). The transport activity was dependent on extracellular pH. In electrophysiological studies, type IIc Na/P(i) cotransport was electroneutral, whereas type IIa was highly electrogenic. In Northern blotting analysis, the type IIc transcript was only expressed in the kidney and highly in weaning animals. In immunohistochemical analysis, the type IIc protein was shown to be localized at the apical membrane of the proximal tubular cells in superficial and midcortical nephrons of weaning rat kidney. Hybrid depletion experiments suggested that type IIc could function as a Na/P(i) cotransporter in weaning animals, but its role is reduced in adults. The finding of the present study suggest that the type IIc is a growth-related renal Na/P(i) cotransporter, which has a high affinity for P(i) and is electroneutral.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc34a3Ratphosphate ion transport  IMP  RGD 
Slc34a1Ratpositive regulation of membrane potential  IDA  RGD 
Slc34a1Ratpositive regulation of sodium-dependent phosphate transport  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc34a3Ratapical plasma membrane  IDA  RGD 
Slc34a3Ratbrush border membrane located_inIDA PMID:11880379UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc34a3Ratsodium:phosphate symporter activity  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc34a1  (solute carrier family 34 member 1)
Slc34a3  (solute carrier family 34 member 3)

Objects referenced in this article
Gene SLC34A3 solute carrier family 34 member 3 Homo sapiens

Additional Information