RGD Reference Report - Rat C1-tetrahydrofolate synthase. cDNA isolation, tissue-specific levels of the mRNA, and expression of the protein in yeast. - Rat Genome Database

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Rat C1-tetrahydrofolate synthase. cDNA isolation, tissue-specific levels of the mRNA, and expression of the protein in yeast.

Authors: Thigpen, AE  West, MG  Appling, DR 
Citation: Thigpen AE, etal., J Biol Chem 1990 May 15;265(14):7907-13.
RGD ID: 1299513
Pubmed: PMID:2186031   (View Abstract at PubMed)

We have isolated and characterized cDNA clones encoding rat cytoplasmic C1-tetrahydrofolate (H4folate) synthase. In eukaryotes, this enzyme is trifunctional and contains the activities of 10-formyl-H4folate synthetase, 5,10-methenyl-H4folate cyclohydrolase, and 5,10-methylene-H4folate dehydrogenase. The deduced sequence of the 935-amino acid open reading frame contained exact matches to NH2-terminal (15 residues) and internal (residues 436-450) peptide sequences obtained from the purified enzyme. The amino acid sequence derived from the rat cDNA shows extensive homology to analogous proteins from bacterial, yeast, and mammalian sources. We have used the cDNA to determine the steady-state levels of the mRNA in various rat tissues and have found that gene expression is regulated in a tissue-specific manner. Transcript levels are highest in kidney and liver with liver transcripts reduced about 30% relative to those found in kidney. Brain, heart, testis, lung and skeletal muscle display even lower transcript levels; reductions range from 70 to 80% of transcript levels found in kidney. Comparison to the levels of enzyme in these tissues allows us to conclude that pretranslational events predominate in the tissue-specific expression. The rat enzyme has been functionally expressed in Saccharomyces cerevisiae as evidenced by its capacity to complement a chromosomal deletion of ADE3, the yeast gene encoding cytoplasmic C1-H4folate synthase.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mthfd1Rattetrahydrofolate metabolic process  TAS  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mthfd1Ratformate-tetrahydrofolate ligase activity  TAS  RGD 
Mthfd1Ratmethenyltetrahydrofolate cyclohydrolase activity  TAS  RGD 
Mthfd1Ratmethylenetetrahydrofolate dehydrogenase [NAD(P)+] activity  TAS  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MTHFD1Humanfolate mediated one-carbon metabolic pathway   ISS  RGD 
Mthfd1Ratfolate mediated one-carbon metabolic pathway   TAS  RGD 
MTHFD1Humanfolate metabolic pathway  ISS  RGD 
Mthfd1Ratfolate metabolic pathway  TAS  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Mthfd1  (methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1)

Genes (Homo sapiens)
MTHFD1  (methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1)


Additional Information