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Interaction of the ERC family of RIM-binding proteins with the liprin-alpha family of multidomain proteins.

Authors: Ko, J  Na, M  Kim, S  Lee, JR  Kim, E 
Citation: Ko J, etal., J Biol Chem 2003 Oct 24;278(43):42377-85. Epub 2003 Aug 15.
Pubmed: (View Article at PubMed) PMID:12923177
DOI: Full-text: DOI:10.1074/jbc.M307561200

Liprin-alpha/SYD-2 is a family of multidomain proteins with four known isoforms. One of the reported functions of liprin-alpha is to regulate the development of presynaptic active zones, but the underlying mechanism is poorly understood. Here we report that liprin-alpha directly interacts with the ERC (ELKS-Rab6-interacting protein-CAST) family of proteins, members of which are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. In vitro results indicate that ERC2/CAST, an active zone-specific isoform, interacts with all of the known isoforms of liprin-alpha and that liprin-alpha1 associates with both ERC2 and ERC1b, a splice variant of ERC1 that distributes to both cytosolic and active zone regions. ERC2 colocalizes with liprin-alpha1 in cultured neurons and forms a complex with liprin-alpha1 in brain. Liprin-alpha1, when expressed alone in cultured neurons, shows a partial synaptic localization. When coexpressed with ERC2, however, liprin-alpha1 is redistributed to synaptic sites. Moreover, roughly the first half of ERC2, which contains the liprin-alpha-binding region, is sufficient for the synaptic localization of liprin-alpha1 while the second half is not. These results suggest that the interaction between ERC2 and liprin-alpha may be involved in the presynaptic localization of liprin-alpha and the molecular organization of presynaptic active zones.

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RGD Object Information
RGD ID: 1299402
Created: 2004-06-01
Species: All species
Last Modified: 2006-04-25
Status: ACTIVE



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