RGD Reference Report - Syntaxin 2 splice variants exhibit differential expression patterns, biochemical properties and subcellular localizations. - Rat Genome Database

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Syntaxin 2 splice variants exhibit differential expression patterns, biochemical properties and subcellular localizations.

Authors: Quinones, B  Riento, K  Olkkonen, VM  Hardy, S  Bennett, MK 
Citation: Quinones B, etal., J Cell Sci 1999 Dec;112 ( Pt 23):4291-304.
RGD ID: 1298910
Pubmed: PMID:10564647   (View Abstract at PubMed)

The syntaxins are a large protein family implicated in the targeting and fusion of intracellular transport vesicles. A subset of proteins of this family are the four syntaxin 2 splice variants, syntaxins 2A (2), 2B (2'), 2C (2") and 2D. Each syntaxin 2 variant contains an identical, or nearly identical, amino-terminal cytoplasmic domain followed by a distinct hydrophobic (syntaxins 2A and 2B) or hydrophilic (syntaxins 2C and 2D) carboxyl-terminal domain. To investigate whether the difference among the syntaxin 2 variants is functionally important, we have examined comparatively their RNA transcript and protein expression patterns, membrane associations, protein-protein interactions and intracellular localizations. Analysis of the RNA transcript and protein expression patterns demonstrated that syntaxins 2A, 2B and 2C are broadly, but not uniformly, expressed while syntaxin 2D expression is restricted to the brain. Subcellular fractionation studies showed that syntaxins 2A and 2B behave as integral membrane proteins while syntaxin 2C is only partially associated with membranes. In vitro biochemical assays demonstrated that the syntaxin 2 variants exhibit similar yet distinct interactions with other proteins implicated in vesicular trafficking, including SNAP-25, SNAP-23, VAMP-2 and n-sec1. In a variety of nonpolarized cell types, syntaxins 2A and 2B localized to both the plasma membrane and endosomal membranes. However, in two polarized epithelial cell lines, MDCK and Caco-2, syntaxin 2A localized predominantly to the apical plasma membrane while syntaxin 2B was associated with both the apical and the basolateral membranes. These observations indicate that the distinct carboxyl-terminal domains of the syntaxin 2 variants influence their biochemical and localization properties and may therefore confer upon these variants different functional roles in the regulation of intracellular membrane trafficking.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
intracellular transport  TAS 1298910 RGD 
vesicle fusion  TAS 1298910 RGD 
vesicle targeting  TAS 1298910 RGD 
vesicle-mediated transport  TAS 1298910 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
apical plasma membrane  IDA 1298910; 1298910 RGD 
basolateral plasma membrane  IDA 1298910 RGD 
endosome  IDA 1298910; 1298910 RGD 
SNARE complex  IDA 1298910; 1298910; 1298910; 1298910 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
SNARE binding  IMP 1298910 RGD 
SNARE binding  IDA 1298910; 1298910; 1298910; 1298910 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Stx2  (syntaxin 2)
Stx2_v1  (syntaxin 2, variant 1)
Stx2_v2  (syntaxin 2, variant 2)
Stx2_v3  (syntaxin 2, variant 3)
Stx2_v4  (syntaxin 2, variant 4)


Additional Information