RGD Reference Report - Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells. - Rat Genome Database

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Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells.

Authors: Low, SH  Li, X  Miura, M  Kudo, N  Quinones, B  Weimbs, T 
Citation: Low SH, etal., Dev Cell 2003 May;4(5):753-9.
RGD ID: 1298908
Pubmed: PMID:12737809   (View Abstract at PubMed)

The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. Here we show that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP-8, specifically localize to the midbody during cytokinesis in mammalian cells. Inhibition of their function by overexpression of nonmembrane-anchored mutants causes failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy shows that only midbody abscission but not further upstream events, such as furrowing, are affected. These results indicate that successful completion of cytokinesis requires a SNARE-mediated membrane fusion event and that this requirement is distinct from exocytic events that may be involved in prior ingression of the plasma membrane.



Gene Ontology Annotations    

Biological Process

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Stx2  (syntaxin 2)
Vamp3  (vesicle-associated membrane protein 3)
Vamp8  (vesicle-associated membrane protein 8)


Additional Information