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Structure of the rat gene encoding cholesterol 7 alpha-hydroxylase.

Authors: Jelinek, DF  Russell, DW 
Citation: Jelinek DF and Russell DW, Biochemistry 1990 Aug 28;29(34):7781-5.
Pubmed: (View Article at PubMed) PMID:2261433

Cholesterol 7 alpha-hydroxylase (7 alpha-hydroxylase) is a microsomal cytochrome P-450 that catalyzes the first and rate-limiting step in bile acid biosynthesis, the major catabolic pathway in cholesterol homeostasis. The gene encoding the rat 7 alpha-hydroxylase has been isolated and characterized. Southern blotting experiments demonstrated that the gene is present in a single copy in the rat genome. DNA sequence analysis showed that the 7 alpha-hydroxylase gene is unique among the characterized cytochrome P-450s in that it contains only six exons. Nuclease S1 and primer-extension mapping experiments positioned the 5'-ends of the 7 alpha-hydroxylase mRNA approximately 20-25 nucleotides downstream of a consensus TATAAA sequence. RNA blotting experiments demonstrated the presence of multiple 7 alpha-hydroxylase mRNAs that differ in the lengths of their 3'-untranslated regions.

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RGD ID: 1298866
Created: 2004-06-01
Species: All species
Last Modified: 2004-06-01
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