RGD Reference Report - Expression of cytochrome CYP2B1/2 in nonpregnant, pregnant and fetal rats exposed to tobacco smoke. - Rat Genome Database

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Expression of cytochrome CYP2B1/2 in nonpregnant, pregnant and fetal rats exposed to tobacco smoke.

Authors: Czekaj, P  Wiaderkiewicz, A  Florek, E  Wiaderkiewicz, R 
Citation: Czekaj P, etal., Acta Biochim Pol 2000;47(4):1115-27.
RGD ID: 1298848
Pubmed: PMID:11996101   (View Abstract at PubMed)

Four-month-old female Wistar rats were exposed for 20 days to tobacco smoke obtained from non-filter cigarettes. During the exposure, concentration of tobacco smoke was monitored indirectly by measuring the CO level (1500 mg/m3 air). The efficacy of exposure was assessed by measuring urine nicotine and cotinine levels. Cigarette smoke did not change total cytochrome P450 and b5 protein levels in any of the organs studied, and most of these organs did not show any changes in the activity of reductases associated with these cytochromes. Following exposure to tobacco smoke, fetal rat liver expressed CYP2B1/2 protein; in newborns (day 1) both liver and lung showed CYP2B1/2 protein expression and very low pentoxyresorufin O-dealkylase activity. Western blot analysis of adult liver, lung, heart, but not of brain microsomes, showed that tobacco smoke induced CYP2B1/2 in both nonpregnant and pregnant rats, though its expression was lower in the livers and hearts of pregnant females. In the rat and human placenta, neither rat CYP2B1/2 nor human CYP2B6 showed basal or tobacco smoke-induced expression at the protein level. This study shows clearly that the expression of CYP2B1/2, which metabolizes nicotine and some drugs and activates carcinogens, is controlled in rats by age-, pregnancy-, and tissue-specific regulatory mechanisms.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cyp2b2Ratnicotine metabolic process  IEP  RGD 
Cyp2b2Ratxenobiotic metabolic process  TAS  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp2b2  (cytochrome P450, family 2, subfamily b, polypeptide 2)

Objects referenced in this article
Gene Cyp2b1 cytochrome P450, family 2, subfamily b, polypeptide 1 Rattus norvegicus

Additional Information