RGD Reference Report - Nitrous oxide exposure reduces hepatic C1-tetrahydrofolate synthase expression in rats. - Rat Genome Database

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Nitrous oxide exposure reduces hepatic C1-tetrahydrofolate synthase expression in rats.

Authors: Barlowe, C K  Appling, D R 
Citation: Barlowe CK and Appling DR, Biochem Biophys Res Commun. 1988 Nov 30;157(1):245-9.
RGD ID: 12914153
Pubmed: PMID:3264158   (View Abstract at PubMed)

C1-tetrahydrofolate synthase (C1-THF synthase) is a trifunctional enzyme which catalyzes the interconversion of one-carbon units attached to the coenzyme THF. Nitrous oxide (N2O) inhalation is known to inactivate hepatic cobalamin-dependent methionine synthase leading to methionine deficiency and trapping of THF in the methyl-THF form. Liver tissue from rats exposed to N2O for 48 hours exhibited a coordinate decrease in all three activities of C1-THF synthase of approximately 25%. A corresponding 25% decrease in immunoreactive C1-THF synthase was also observed after 48 hours. Thus, the decrease in the concentration of C1-THF synthase accounted entirely for the decreases observed in the three activities. These results suggest that perturbations of hepatic THF pools by N2O affect the level of C1-THF synthase expression at a translational or pretranslational level.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to nitrogen dioxide  IEP 12914153 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mthfd1  (methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1)


Additional Information