RGD Reference Report - Preliminary Characterization of a Leptin Receptor Knockout Rat Created by CRISPR/Cas9 System. - Rat Genome Database

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Preliminary Characterization of a Leptin Receptor Knockout Rat Created by CRISPR/Cas9 System.

Authors: Bao, Dan  Ma, Yuanwu  Zhang, Xu  Guan, Feifei  Chen, Wei  Gao, Kai  Qin, Chuan  Zhang, Lianfeng 
Citation: Bao D, etal., Sci Rep. 2015 Nov 5;5:15942. doi: 10.1038/srep15942.
RGD ID: 12911216
Pubmed: PMID:26537785   (View Abstract at PubMed)
PMCID: PMC4633582   (View Article at PubMed Central)
DOI: DOI:10.1038/srep15942   (Journal Full-text)

Leptin receptor, which is encoded by the diabetes (db) gene and is highly expressed in the choroid plexus, regulatesenergy homeostasis, the balance between food intake and energy expenditure, fertility and bone mass. Here, using CRISPR/Cas9 technology, we created the leptin receptor knockout rat. Homozygous leptin receptor null rats are characterized by obesity, hyperphagia, hyperglycemia, glucose intolerance, hyperinsulinemia and dyslipidemia. Due to long-term poor glycemic control, the leptin receptor knockout rats also develop some diabetic complications such as pancreatic, hepatic and renal lesions. In addition, the leptin receptor knockout rats show a significant decrease in bone volume and bone mineral density of the femur compared with their wild-type littermates. Our model has rescued some deficiency of the existing rodent models, such as the transient hyperglycemia of db/db mice in the C57BL/6J genetic background and the delayed onset of glucose intolerance in the Zucker rats, and it is proven to be a useful animal model for biomedical and pharmacological research on obesity and diabetes.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
LeprRatdecreased bone mineral density  IMP  RGD 
Leprem4LizhRatdecreased bone mineral density  IMP  RGD 
SD-Leprem4LizhRatdecreased bone mineral density  IMP  RGD 
LeprRatdecreased trabecular bone volume  IMP  RGD 
Leprem4LizhRatdecreased trabecular bone volume  IMP  RGD 
SD-Leprem4LizhRatdecreased trabecular bone volume  IMP  RGD 
LeprRatexpanded mesangial matrix  IMP  RGD 
Leprem4LizhRatexpanded mesangial matrix  IMP  RGD 
SD-Leprem4LizhRatexpanded mesangial matrix  IMP  RGD 
LeprRathepatic steatosis  IMP  RGD 
Leprem4LizhRathepatic steatosis  IMP  RGD 
SD-Leprem4LizhRathepatic steatosis  IMP  RGD 
LeprRatincreased bone trabecular spacing  IMP  RGD 
Leprem4LizhRatincreased bone trabecular spacing  IMP  RGD 
SD-Leprem4LizhRatincreased bone trabecular spacing  IMP  RGD 
LeprRatincreased circulating cholesterol level  IMP  RGD 
Leprem4LizhRatincreased circulating cholesterol level  IMP compared to wild type littermateRGD 
SD-Leprem4LizhRatincreased circulating cholesterol level  IMP compared to wild type littermateRGD 
LeprRatincreased circulating HDL cholesterol level  IMP  RGD 
Leprem4LizhRatincreased circulating HDL cholesterol level  IMP compared to wild type littermateRGD 
SD-Leprem4LizhRatincreased circulating HDL cholesterol level  IMP compared to wild type littermateRGD 
LeprRatincreased circulating LDL cholesterol level  IMP  RGD 
Leprem4LizhRatincreased circulating LDL cholesterol level  IMP compared to wild type littermateRGD 
SD-Leprem4LizhRatincreased circulating LDL cholesterol level  IMP compared to wild type littermateRGD 
LeprRatincreased circulating triglyceride level  IMP  RGD 
Leprem4LizhRatincreased circulating triglyceride level  IMP compared to wild type littermateRGD 
SD-Leprem4LizhRatincreased circulating triglyceride level  IMP compared to wild type littermateRGD 
LeprRatincreased fat cell size  IMP  RGD 
Leprem4LizhRatincreased fat cell size  IMP  RGD 
SD-Leprem4LizhRatincreased fat cell size  IMP  RGD 
LeprRatpancreas fibrosis  IMP  RGD 
Leprem4LizhRatpancreas fibrosis  IMP  RGD 
SD-Leprem4LizhRatpancreas fibrosis  IMP  RGD 
LeprRatpancreas hyperplasia  IMP  RGD 
Leprem4LizhRatpancreas hyperplasia  IMP  RGD 
SD-Leprem4LizhRatpancreas hyperplasia  IMP  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Lepr  (leptin receptor)
Leprem4Lizh  (leptin receptor; CRISPR/Cas9 induced mutant 4, Lizh)

Genes (Mus musculus)
Lepr  (leptin receptor)

Genes (Homo sapiens)
LEPR  (leptin receptor)

Strains
SD-Leprem4Lizh  (NA)


Additional Information