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Characterization of Dp71¿(78-79), a novel dystrophin mutant that stimulates PC12 cell differentiation.

Authors: Aragón, Jorge  Romo-Yáñez, José  Martínez-Herrera, Alejandro  Ceja, Víctor  Rendon, Alvaro  Montañez, Cecilia 
Citation: Aragón J, etal., J Neurochem. 2011 Nov;119(4):697-707. doi: 10.1111/j.1471-4159.2011.07347.x. Epub 2011 Sep 22.
Pubmed: (View Article at PubMed) PMID:21668890
DOI: Full-text: DOI:10.1111/j.1471-4159.2011.07347.x

Dp71 has an important role in the central nervous system. To better understand the function of Dp71 domains in neuronal differentiation, PC12 cells were stably transfected with a dystrophin mutant, Dp71¿(78-79) , which lacks exons 78 and 79. Based on the percentage of cells bearing neurites and neurite length analyses, we found that cells stably expressing Dp71¿(78-79) (PC12-C11) differentiate more efficiently than non-transfected cells. While wild-type cells reach their maximum differentiation 9-12 days after initiating the differentiation process, the PC12-C11 cells reach differentiation in 4-6 days. Protein expression analysis showed a down-regulation of Dp71a and an up-regulation of Dp71ab and/or Up71, ß-dystroglycan and neuron-specific enolase in undifferentiated and in neural growth factor differentiated PC12-C11 cells. No change was observed in the expression of Grb2 and Up400. The subcellular localization of Dp71¿(78-79) was in the cell periphery, and there was no change in localization during the differentiation process, which was also observed throughout the neurite extensions.

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RGD ID: 12902619
Created: 2017-05-09
Species: All species
Last Modified: 2017-05-09
Status: ACTIVE



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