RGD Reference Report - Regulation of dopamine presynaptic markers and receptors in the striatum of DJ-1 and Pink1 knockout rats. - Rat Genome Database

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Regulation of dopamine presynaptic markers and receptors in the striatum of DJ-1 and Pink1 knockout rats.

Authors: Sun, Jianjun  Kouranova, Evguenia  Cui, Xiaoxia  Mach, Robert H  Xu, Jinbin 
Citation: Sun J, etal., Neurosci Lett. 2013 Dec 17;557 Pt B:123-8. doi: 10.1016/j.neulet.2013.10.034. Epub 2013 Oct 22.
RGD ID: 12880446
Pubmed: PMID:24157858   (View Abstract at PubMed)
PMCID: PMC4144334   (View Article at PubMed Central)
DOI: DOI:10.1016/j.neulet.2013.10.034   (Journal Full-text)

Pathogenic autosomal recessive mutations in the DJ-1 (Park7) or the PTEN-induced putative kinase 1 (Pink1 or PARK6) genes are associated with familial Parkinson's disease (PD). It is not well known regarding the pathological mechanisms involving the DJ-1 and Pink1 mutations. Here we characterized DJ-1 and Pink1 knockout rats both through expression profiling and using quantitative autoradiography to measure the densities of the dopamine D1, D2, D3 receptors, vesicular monoamine transporter type-2 (VMAT2) and dopamine transporter (DAT) in the striatum of transgenic rats and wild type controls. Expression profiling with a commercially available array of 84 genes known to be involved in PD indicated that only the target gene was significantly downregulated in each transgenic rat model. D1 receptor, VMAT2, and DAT were measured using [(3)H]SCH23390, [(3)H]dihydrotetrabenazine, and [(3)H]WIN35428, respectively. No significant changes were observed in the density of DAT in either model. Although the densities of VMAT2 and D1 receptor were unchanged in Pink1 knockout, but both were increased in DJ-1 knockout rats. The densities of D2 and D3 receptors, determined by mathematical analysis of binding of radioligands [(3)H]WC-10 and [(3)H]raclopride, were significantly increased in both knockout models. These distinctive changes in the expression of dopamine presynaptic markers and receptors in the striatum may reflect different compensatory regulation of dopamine system in DJ-1 versus Pink1 knockout rat models of familial PD.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Objects Annotated

Genes (Rattus norvegicus)
Park7  (Parkinsonism associated deglycase)
Park7em1Sage  (parkinson protein 7; zinc finger nuclease induced mutant 1, Sigma Advanced Genetic Engineering Labs)
Pink1  (PTEN induced kinase 1)
Pink1em1Sage  (PTEN induced putative kinase 1; zinc finger nuclease induced mutant 1, Sigma Advanced Genetic Engineering Labs)

Genes (Mus musculus)
Park7  (Parkinson disease (autosomal recessive, early onset) 7)
Pink1  (PTEN induced putative kinase 1)

Genes (Homo sapiens)
PARK7  (Parkinsonism associated deglycase)
PINK1  (PTEN induced kinase 1)


Additional Information