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Positioning of AMPA Receptor-Containing Endosomes Regulates Synapse Architecture.

Authors: Esteves da Silva, Marta  Adrian, Max  Sch√§tzle, Philipp  Lipka, Joanna  Watanabe, Takuya  Cho, Sukhee  Futai, Kensuke  Wierenga, Corette J  Kapitein, Lukas C  Hoogenraad, Casper C 
Citation: Esteves da Silva M, etal., Cell Rep. 2015 Nov 3;13(5):933-43. doi: 10.1016/j.celrep.2015.09.062. Epub 2015 Oct 22.
Pubmed: (View Article at PubMed) PMID:26565907
DOI: Full-text: DOI:10.1016/j.celrep.2015.09.062

Lateral diffusion in the membrane and endosomal trafficking both contribute to the addition and removal of AMPA receptors (AMPARs) at postsynaptic sites. However, the spatial coordination between these mechanisms has remained unclear, because little is known about the dynamics of AMPAR-containing endosomes. In addition, how the positioning of AMPAR-containing endosomes affects synapse organization and functioning has never been directly explored. Here, we used live-cell imaging in hippocampal neuron cultures to show that intracellular AMPARs are transported in Rab11-positive recycling endosomes, which frequently enter dendritic spines and depend on the microtubule and actin cytoskeleton. By using chemically induced dimerization systems to recruit kinesin (KIF1C) or myosin (MyosinV/VI) motors to Rab11-positive recycling endosomes, we controlled their trafficking and found that induced removal of recycling endosomes from spines decreases surface AMPAR expression and PSD-95 clusters at synapses. Our data suggest a mechanistic link between endosome positioning and postsynaptic structure and composition.


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RGD Object Information
RGD ID: 12859075
Created: 2017-04-12
Species: All species
Last Modified: 2017-04-12
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.