RGD Reference Report - Molecular architecture and functional model of the endocytic AP2 complex. - Rat Genome Database

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Molecular architecture and functional model of the endocytic AP2 complex.

Authors: Collins, Brett M  McCoy, Airlie J  Kent, Helen M  Evans, Philip R  Owen, David J 
Citation: Collins BM, etal., Cell. 2002 May 17;109(4):523-35.
RGD ID: 12793054
Pubmed: PMID:12086608   (View Abstract at PubMed)

AP2 is the best-characterized member of the family of heterotetrameric clathrin adaptor complexes that play pivotal roles in many vesicle trafficking pathways within the cell. AP2 functions in clathrin-mediated endocytosis, the process whereby cargo enters the endosomal system from the plasma membrane. We describe the structure of the 200 kDa AP2 "core" (alpha trunk, beta2 trunk, mu2, and sigma2) complexed with the polyphosphatidylinositol headgroup mimic inositolhexakisphosphate at 2.6 A resolution. Two potential polyphosphatidylinositide binding sites are observed, one on alpha and one on mu2. The binding site for Yxxphi endocytic motifs is buried, indicating that a conformational change, probably triggered by phosphorylation in the disordered mu2 linker, is necessary to allow Yxxphi motif binding. A model for AP2 recruitment and activation is proposed.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ap2a2RatAP-2 adaptor complex part_ofIMP PMID:12086608CAFA 
Ap2m1RatAP-2 adaptor complex part_ofIDA PMID:12086608CAFA 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ap2a2Ratphosphatidylinositol binding enablesIMP PMID:12086608CAFA 
Ap2a2Ratprotein binding enablesIPIUniProtKB:P62743 more ...PMID:12086608CAFA 
Ap2m1Ratprotein binding enablesIPIUniProtKB:P18484 more ...PMID:12086608CAFA 

Objects Annotated

Genes (Rattus norvegicus)
Ap2a2  (adaptor related protein complex 2 subunit alpha 2)
Ap2m1  (adaptor related protein complex 2 subunit mu 1)


Additional Information