RGD Reference Report - Dynein light chain regulates axonal trafficking and synaptic levels of Bassoon. - Rat Genome Database

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Dynein light chain regulates axonal trafficking and synaptic levels of Bassoon.

Authors: Fejtova, Anna  Davydova, Daria  Bischof, Ferdinand  Lazarevic, Vesna  Altrock, Wilko D  Romorini, Stefano  Schöne, Cornelia  Zuschratter, Werner  Kreutz, Michael R  Garner, Craig C  Ziv, Noam E  Gundelfinger, Eckart D 
Citation: Fejtova A, etal., J Cell Biol. 2009 Apr 20;185(2):341-55. doi: 10.1083/jcb.200807155.
RGD ID: 12793047
Pubmed: PMID:19380881   (View Abstract at PubMed)
PMCID: PMC2700376   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200807155   (Journal Full-text)

Bassoon and the related protein Piccolo are core components of the presynaptic cytomatrix at the active zone of neurotransmitter release. They are transported on Golgi-derived membranous organelles, called Piccolo-Bassoon transport vesicles (PTVs), from the neuronal soma to distal axonal locations, where they participate in assembling new synapses. Despite their net anterograde transport, PTVs move in both directions within the axon. How PTVs are linked to retrograde motors and the functional significance of their bidirectional transport are unclear. In this study, we report the direct interaction of Bassoon with dynein light chains (DLCs) DLC1 and DLC2, which potentially link PTVs to dynein and myosin V motor complexes. We demonstrate that Bassoon functions as a cargo adapter for retrograde transport and that disruption of the Bassoon-DLC interactions leads to impaired trafficking of Bassoon in neurons and affects the distribution of Bassoon and Piccolo among synapses. These findings reveal a novel function for Bassoon in trafficking and synaptic delivery of active zone material.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axo-dendritic transport involved_inIDA 12793047PMID:19380881SynGO-UCL 
retrograde axonal transport involved_inIDA 12793047PMID:19380881SynGO-UCL 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
axon cytoplasm located_inIDA 12793047; 12793047PMID:19380881SynGO-UCL 
cell cortex located_inIDA 12793047PMID:19380881SynGO-UCL 
piccolo-bassoon transport vesicle located_inIDA 12793047; 12793047PMID:19380881SynGO-UCL 
synaptic membrane located_inIDA 12793047PMID:19380881SynGO-UCL 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Bsn  (bassoon (presynaptic cytomatrix protein))
Dync1i1  (dynein cytoplasmic 1 intermediate chain 1)
Dynll1  (dynein light chain LC8-type 1)
Dynll2  (dynein light chain LC8-type 2)


Additional Information