RGD Reference Report - Unusual structural organization of the endocytic proteins AP180 and epsin 1. - Rat Genome Database

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Unusual structural organization of the endocytic proteins AP180 and epsin 1.

Authors: Kalthoff, Christoph  Alves, J├╝rgen  Urbanke, Claus  Knorr, Ruth  Ungewickell, Ernst J 
Citation: Kalthoff C, etal., J Biol Chem. 2002 Mar 8;277(10):8209-16. Epub 2001 Dec 26.
RGD ID: 12793027
Pubmed: PMID:11756460   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M111587200   (Journal Full-text)

Epsin and AP180/CALM are important endocytic accessory proteins that are believed to be involved in the formation of clathrin coats. Both proteins associate with phosphorylated membrane inositol lipids through their epsin N-terminal homology domains and with other components of the endocytic machinery through short peptide motifs in their carboxyl-terminal segments. Using hydrodynamic and spectroscopic methods, we demonstrate that the parts of epsin 1 and AP180 that are involved in protein-protein interactions behave as poorly structured flexible polypeptide chains with little or no conventional secondary structure. The predominant cytosolic forms of both proteins are monomers. Furthermore, we show that recombinant epsin 1, like AP180, drives in vitro assembly of clathrin cages. We conclude that the epsin N-terminal homology domain-containing proteins AP180/CALM and epsin 1 have a very similar molecular architecture that is designed for the rapid and efficient recruitment of the principal coat components clathrin and AP-2 at the sites of coated pit assembly.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
clathrin coat assembly involved_inIDA 12793027; 12793027PMID:11756460DisProt 
positive regulation of clathrin coat assembly involved_inIMP 12793027; 12793027PMID:11756460CAFA 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cytosol located_inIDA 12793027; 12793027PMID:11756460CAFA 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Epn1  (Epsin 1)
Snap91  (synaptosome associated protein 91)

Additional Information