RGD Reference Report - The monomer of pyruvate kinase, subtype M1, is both a kinase and a cytosolic thyroid hormone binding protein. - Rat Genome Database

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The monomer of pyruvate kinase, subtype M1, is both a kinase and a cytosolic thyroid hormone binding protein.

Authors: Parkison, C  Ashizawa, K  McPhie, P  Lin, K H  Cheng, S Y 
Citation: Parkison C, etal., Biochem Biophys Res Commun. 1991 Aug 30;179(1):668-74.
RGD ID: 12793012
Pubmed: PMID:1883388   (View Abstract at PubMed)

Using a T7 expression system, the monomer of rat pituitary pyruvate kinase, subtype M1 (PKM1), was overexpressed in Escherichia coli and purified to homogeneity. The monomeric p58-M1 has intrinsic enzymatic activity with a Vmax of 79 +/- 20 units/mg and Km's for ADP and PEP of 1.43 +/- 0.76 and 0.14 +/- 0.07 mM, respectively. The monomer binds 3,3',5-triiodo-L-thyronine (T3) with Ka = 1.5 x 10(7) M-1. The order of analog specificity is L-T3 greater than L-thyroxine greater than D-T3 greater than 3'-isopropyl-3,5-diiodo-L-thyronine greater than or equal to 3',5',3-triiodo-L-thyronine. In contrast, tetrameric PKM1 lacks T3 binding activity. The kinase activity of p58-M1 is inhibited by T3 and its analogs in a concentration-dependent manner with the order of inhibitory activity similar to that of binding activity. This inhibition, however, is reversed by the addition of fructose 1,6-bisphosphate. p58-M1 is the second PK isoenzyme monomer to be identified as having thyroid hormone binding activity.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PkmRatglycolytic process involved_inIDA PMID:1883388CAFA 
Pkm_v1Ratglycolytic process involved_inIDA PMID:1883388CAFA 
Pkm_v2Ratglycolytic process involved_inIDA PMID:1883388CAFA 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PkmRatpyruvate kinase complex part_ofIDA PMID:1883388CAFA 
Pkm_v1Ratpyruvate kinase complex part_ofIDA PMID:1883388CAFA 
Pkm_v2Ratpyruvate kinase complex part_ofIDA PMID:1883388CAFA 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Pkm  (pyruvate kinase M1/2)
Pkm_v1  (pyruvate kinase M1/2, variant 1)
Pkm_v2  (pyruvate kinase M1/2, variant 2)


Additional Information