RGD Reference Report - Characterization of a novel lysozyme-like 4 gene in the rat.

General

Characterization of a novel lysozyme-like 4 gene in the rat.
Authors:	Narmadha, Ganapathy Muneswararao, Katakam Rajesh, Angireddy Yenugu, Suresh
Citation:	Narmadha G, etal., PLoS One. 2011;6(11):e27659. doi: 10.1371/journal.pone.0027659. Epub 2011 Nov 15.
RGD ID:	12793004
Pubmed:	PMID:22110709 (View Abstract at PubMed)
PMCID:	PMC3217008 (View Article at PubMed Central)
DOI:	DOI:10.1371/journal.pone.0027659 (Journal Full-text)
Lysozyme-like proteins (LYZLs) belong to the class of c-type lysozymes and are not well characterized in many species including the rat. In this study, using in silico and molecular biology techniques, we report the identification, cloning and characterization of rat Lyzl4 gene and also determine the expression pattern of Lyzl1, Lyzl3 and Lyzl6. The rat Lyzl genes were found to be distributed on three chromosomes and all of them retained the characteristic eight cysteine signature of c-type lysozyme. Homology modeling of rat LYZL4 indicated that its structure is similar to that of the mouse SLLP1. In the male reproductive tract of rat, Lyzl gene expression was confined to the testis. Lyzl1 and Lyzl4 were found to be expressed in tissues beyond the male reproductive tract, whereas Lyzl3 and Lyzl6 were not. Lyzl expression in the developing (10-60 day old) rats was androgen dependent in the testis. Immunodetection using antibodies against rat LYZL4 revealed the presence of LYZL4 protein in the germinal layer of the testes and on the sperm tail. Recombinant LYZL4 did not exhibit antibacterial, muramidase and isopeptidase activities characteristic to c-type lysozyme. To the best of our knowledge, for the first time we report the characterization of Lyzl genes in the rat. Results of our study indicate that rat LYZL proteins may have an important role in male reproductive tract function.

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Biological Process

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
defense response to Gram-negative bacterium	NOT\|involved_in	IDA		12793004	PMID:22110709	UniProt

Cellular Component

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
sperm flagellum	located_in	IDA		12793004	PMID:22110709	UniProt

Molecular Function

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
lysozyme activity	NOT\|enables	IDA		12793004	PMID:22110709	UniProt

Objects Annotated

Genes (Rattus norvegicus)

Lyzl4 (lysozyme-like 4)

Additional Information

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Characterization of a novel lysozyme-like 4 gene in the rat.