RGD Reference Report - Curcumin prevents mitochondrial dynamics disturbances in early 5/6 nephrectomy: Relation to oxidative stress and mitochondrial bioenergetics. - Rat Genome Database

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Curcumin prevents mitochondrial dynamics disturbances in early 5/6 nephrectomy: Relation to oxidative stress and mitochondrial bioenergetics.

Authors: Aparicio-Trejo, Omar Emiliano  Tapia, Edilia  Molina-Jijón, Eduardo  Medina-Campos, Omar Noel  Macías-Ruvalcaba, Norma Angélica  León-Contreras, Juan Carlos  Hernández-Pando, Rogelio  García-Arroyo, Fernando E  Cristóbal, Magdalena  Sánchez-Lozada, Laura Gabriela  Pedraza-Chaverri, José 
Citation: Aparicio-Trejo OE, etal., Biofactors. 2016 Nov 1. doi: 10.1002/biof.1338.
RGD ID: 12738369
Pubmed: PMID:27801955   (View Abstract at PubMed)
DOI: DOI:10.1002/biof.1338   (Journal Full-text)

Five-sixths nephrectomy (5/6NX) is a widely used model to study the mechanisms leading to renal damage in chronic kidney disease (CKD). However, early alterations on renal function, mitochondrial dynamics, and oxidative stress have not been explored yet. Curcumin is an antioxidant that has shown nephroprotection in 5/6NX-induced renal damage. The aim of this study was to explore the effect of curcumin on early mitochondrial alterations induced by 5/6NX in rats. In isolated mitochondria, 5/6NX-induced hydrogen peroxide production was associated with decreased activity of complexes I and V, decreased activity of antioxidant enzymes, alterations in oxygen consumption and increased MDA-protein adducts. In addition, it was found that 5/6NX shifted mitochondrial dynamics to fusion, which was evidenced by increased optic atrophy 1 and mitofusin 1 (Mfn1) and decreased fission 1 and dynamin-related protein 1 expressions. These data were confirmed by morphological analysis and immunoelectron microscopy of Mfn-1. All the above-described mechanisms were prevented by curcumin. Also, it was found that curcumin prevented renal dysfunction by improving renal blood flow and the total antioxidant capacity induced by 5/6NX. Moreover, in glomeruli and proximal tubules 5/6NX-induced superoxide anion production by uncoupled nitric oxide synthase (NOS) and nicotinamide adenine dinucleotide phosphate oxidase (NOX) dependent way, this latter was associated with increased phosphorylation of serine 304 of p47phox subunit of NOX. In conclusion, this study shows that curcumin pretreatment decreases early 5/6NX-induced altered mitochondrial dynamics, bioenergetics, and oxidative stress, which may be associated with the preservation of renal function. © 2016 BioFactors, 00(00):000000, 2016.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Opa1Ratresponse to curcumin  IEP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Mfn1Ratmitochondrial inner membrane  IDA  RGD 
Mfn1Ratmitochondrial outer membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dnm1l  (dynamin 1-like)
Fis1  (fission, mitochondrial 1)
Mfn1  (mitofusin 1)
Opa1  (OPA1, mitochondrial dynamin like GTPase)

Genes (Mus musculus)
Dnm1l  (dynamin 1-like)
Fis1  (fission, mitochondrial 1)
Mfn1  (mitofusin 1)
Opa1  (OPA1, mitochondrial dynamin like GTPase)

Genes (Homo sapiens)
DNM1L  (dynamin 1 like)
FIS1  (fission, mitochondrial 1)
MFN1  (mitofusin 1)
OPA1  (OPA1 mitochondrial dynamin like GTPase)


Additional Information