RGD Reference Report - Post-sepsis immunosuppression depends on NKT cell regulation of mTOR/IFN-γ in NK cells. - Rat Genome Database

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Post-sepsis immunosuppression depends on NKT cell regulation of mTOR/IFN-γ in NK cells.

Authors: Kim, Edy Y  Ner-Gaon, Hadas  Varon, Jack  Cullen, Aidan M  Guo, Jingyu  Choi, Jiyoung  Barragan-Bradford, Diana  Higuera, Angelica  Pinilla-Vera, Mayra  Short, Samuel Ap  Arciniegas-Rubio, Antonio  Tamura, Tomoyoshi  Leaf, David E  Baron, Rebecca M  Shay, Tal  Brenner, Michael B 
Citation: Kim EY, etal., J Clin Invest. 2020 Jun 1;130(6):3238-3252. doi: 10.1172/JCI128075.
RGD ID: 127345119
Pubmed: PMID:32154791   (View Abstract at PubMed)
PMCID: PMC7260006   (View Article at PubMed Central)
DOI: DOI:10.1172/JCI128075   (Journal Full-text)

As treatment of the early, inflammatory phase of sepsis improves, post-sepsis immunosuppression and secondary infection have increased in importance. How early inflammation drives immunosuppression remains unclear. Although IFN-γ typically helps microbial clearance, we found that increased plasma IFN-γ in early clinical sepsis was associated with the later development of secondary Candida infection. Consistent with this observation, we found that exogenous IFN-γ suppressed macrophage phagocytosis of zymosan in vivo, and antibody blockade of IFN-γ after endotoxemia improved survival of secondary candidemia. Transcriptomic analysis of innate lymphocytes during endotoxemia suggested that NKT cells drove IFN-γ production by NK cells via mTORC1. Activation of invariant NKT (iNKT) cells with glycolipid antigen drove immunosuppression. Deletion of iNKT cells in Cd1d-/- mice or inhibition of mTOR by rapamycin reduced immunosuppression and susceptibility to secondary Candida infection. Thus, although rapamycin is typically an immunosuppressive medication, in the context of sepsis, rapamycin has the opposite effect. These results implicated an NKT cell/mTOR/IFN-γ axis in immunosuppression following endotoxemia or sepsis. In summary, in vivo iNKT cells activated mTORC1 in NK cells to produce IFN-γ, which worsened macrophage phagocytosis, clearance of secondary Candida infection, and mortality.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Candidemia amelioratesISOCd1d1 (Mus musculus)127345119; 127345119associated with endotoxemiaRGD 
Candidemia amelioratesIMP 127345119associated with endotoxemiaRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cd1d1  (CD1d1 molecule)

Genes (Mus musculus)
Cd1d1  (CD1d1 antigen)

Genes (Homo sapiens)
CD1D  (CD1d molecule)


Additional Information