RGD Reference Report - Invariant NKT cell response to dengue virus infection in human. - Rat Genome Database

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Invariant NKT cell response to dengue virus infection in human.

Authors: Matangkasombut, Ponpan  Chan-In, Wilawan  Opasawaschai, Anunya  Pongchaikul, Pisut  Tangthawornchaikul, Nattaya  Vasanawathana, Sirijitt  Limpitikul, Wannee  Malasit, Prida  Duangchinda, Thaneeya  Screaton, Gavin  Mongkolsapaya, Juthathip 
Citation: Matangkasombut P, etal., PLoS Negl Trop Dis. 2014 Jun 19;8(6):e2955. doi: 10.1371/journal.pntd.0002955. eCollection 2014 Jun.
RGD ID: 127345113
Pubmed: (View Article at PubMed) PMID:24945350
DOI: Full-text: DOI:10.1371/journal.pntd.0002955


BACKGROUND: Dengue viral infection is a global health threat without vaccine or specific treatment. The clinical outcome varies from asymptomatic, mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). While adaptive immune responses were found to be detrimental in the dengue pathogenesis, the roles of earlier innate events remain largely uninvestigated. Invariant natural killer T (iNKT) cells represent innate-like T cells that could dictate subsequent adaptive response but their role in human dengue virus infection is not known. We hypothesized that iNKT cells play a role in human dengue infection.
METHODS: Blood samples from a well-characterized cohort of children with DF, DHF, in comparison to non-dengue febrile illness (OFI) and healthy controls at various time points were studied. iNKT cells activation were analyzed by the expression of CD69 by flow cytometry. Their cytokine production was then analyzed after α-GalCer stimulation. Further, the CD1d expression on monocytes, and CD69 expression on conventional T cells were measured.
RESULTS: iNKT cells were activated during acute dengue infection. The level of iNKT cell activation associates with the disease severity. Furthermore, these iNKT cells had altered functional response to subsequent ex vivo stimulation with α-GalCer. Moreover, during acute dengue infection, monocytic CD1d expression was also upregulated and conventional T cells also became activated.
CONCLUSION: iNKT cells might play an early and critical role in the pathogenesis of severe dengue viral infection in human. Targeting iNKT cells and CD1d serve as a potential therapeutic strategy for severe dengue infection in the future.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Cd1d1  (CD1d1 molecule)

Genes (Mus musculus)
Cd1d1  (CD1d1 antigen)

Genes (Homo sapiens)
CD1D  (CD1d molecule)


Additional Information