RGD Reference Report - Mucosal type mast cells express complement receptor type 2 (CD21). - Rat Genome Database

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Mucosal type mast cells express complement receptor type 2 (CD21).

Authors: Andrásfalvy, Márton  Prechl, József  Hardy, Tímea  Erdei, Anna  Bajtay, Zsuzsa 
Citation: Andrásfalvy M, etal., Immunol Lett. 2002 Jun 3;82(1-2):29-34. doi: 10.1016/s0165-2478(02)00015-9.
RGD ID: 127338250
Pubmed: PMID:12008031   (View Abstract at PubMed)
DOI: DOI:10.1016/s0165-2478(02)00015-9   (Journal Full-text)

Fragments of complement component C3 generated upon activation of the cascade play an important role in the induction and regulation of immune responses. Receptors interacting with various fragments of this versatile complement protein are expressed on a wide variety of cell types, including lymphocytes, macrophages, dendritic cells, follicular dendritic cells, granulocytes, erythrocytes and consequently, C3-products may influence several biological functions at different sites of the body, where complement activation occurs. Regarding the expression of various C3-receptors on mast cells, mainly rodent serosal type mastocytes have been investigated so far. It has been known for a long time that C3a triggers the release of mediators of immediate type hypersensitivity via binding to serosal-type cells. Complement receptor type 1 (CR1/CD35) and type 2 (CR2/CD21) interacting with the larger activation products, such as C3b and C3d, have so far been shown on serosal type mast cells only. In this study, the expression of CR1/2 on mucosal type mast cells is demonstrated. Using mouse CR1/2 specific single chain antibodies and the natural ligand C3d in cytofluorimetric measurements, we show that the rat mucosal mast cell line RBL-2H3 and mouse bone marrow-derived mast cells (BMMC) express CD21. RT-PCR experiments carried out with mouse CR1 and CR2 specific primers show CD21, but not CD35 specific products in BMMC. It is also demonstrated that, in contrast to serosal type mast cells, mucosal mastocytes do not express CD19. In an attempt to reveal the possible function of CR2 on mucosal type mast cells, the effect of receptor-clustering was tested regarding degranulation, Ca-response and IL-6 production, but no CR2-mediated change was detected in any of these processes.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell surface  IDA 127338250MMO:0000617RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cr2  (complement C3d receptor 2)


Additional Information