RGD Reference Report - Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163. - Rat Genome Database

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Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163.

Authors: Chen, Jingyao  Wang, Haitao  Bai, Jianhui  Liu, Wenjie  Liu, Xiaojuan  Yu, Dawei  Feng, Tao  Sun, Zhaolin  Zhang, Linlin  Ma, Linyuan  Hu, Yiqing  Zou, Yunlong  Tan, Tan  Zhong, Jie  Hu, Man  Bai, Xiaofei  Pan, Dengke  Xing, Yiming  Zhao, Yaofeng  Tian, Kegong  Hu, Xiaoxiang  Li, Ning 
Citation: Chen J, etal., Int J Biol Sci. 2019 Jan 1;15(2):481-492. doi: 10.7150/ijbs.25862. eCollection 2019.
RGD ID: 127285677
Pubmed: (View Article at PubMed) PMID:30745836
DOI: Full-text: DOI:10.7150/ijbs.25862

Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease and the most economically important disease of the swine industry worldwide. Highly pathogenic-PRRS virus (HP-PRRSV) is a variant of PRRSV, which caused high morbidity and mortality. Scavenger receptor CD163, which contains nine scavenger receptor cysteine-rich (SRCR) domains, is a key entry mediator for PRRSV. A previous study demonstrated that SRCR domain 5 (SRCR5), encoded by exon 7, was essential for PRRSV infection in vitro. Here, we substituted exon 7 of porcine CD163 with the corresponding exon of human CD163-like 1 (hCD163L1) using a CRISPR/Cas9 system combined with a donor vector. In CD163Mut/Mut pigs, modifying CD163 gene had no adverse effects on hemoglobin-haptoglobin (Hb-Hp) complex clearance or erythroblast growth. In vitro infection experiments showed that the CD163 mutant strongly inhibited HP-PRRSV replication by inhibiting virus uncoating and genome release. Compared to wild-type (WT) pigs in vivo, HP-PRRSV-infected CD163Mut/Mut pigs showed a substantially decreased viral load in blood and relief from PRRSV-induced fever. While all WT pigs were dead, there of four CD163Mut/Mut pigs survived and recovered at the termination of the experiment. Our data demonstrated that modifying CD163 remarkably inhibited PRRSV replication and protected pigs from HP-PRRSV infection, thus establishing a good foundation for breeding PRRSV-resistant pigs via gene editing technology.

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