RGD Reference Report - Association of Inflammatory Responses and ECM Disorganization with HMGB1 Upregulation and NLRP3 Inflammasome Activation in the Injured Rotator Cuff Tendon. - Rat Genome Database

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Association of Inflammatory Responses and ECM Disorganization with HMGB1 Upregulation and NLRP3 Inflammasome Activation in the Injured Rotator Cuff Tendon.

Authors: Thankam, Finosh G  Roesch, Zachary K  Dilisio, Matthew F  Radwan, Mohamed M  Kovilam, Anuradha  Gross, R Michael  Agrawal, Devendra K 
Citation: Thankam FG, etal., Sci Rep. 2018 Jun 11;8(1):8918. doi: 10.1038/s41598-018-27250-2.
RGD ID: 127284862
Pubmed: (View Article at PubMed) PMID:29891998
DOI: Full-text: DOI:10.1038/s41598-018-27250-2

Inflammation and extracellular matrix (ECM) disorganization following the rotator cuff tendon injuries (RCTI) delay the repair and healing process and the molecular mechanisms underlying RCTI pathology are largely unknown. Here, we examined the role of HMGB1 and NLRP3 inflammasome pathway in the inflammation and ECM disorganization in RCTI. This hypothesis was tested in a tenotomy-RCTI rat model by transecting the RC tendon from the humerus. H&E and pentachrome staining revealed significant changes in the morphology, architecture and ECM organization in RC tendon tissues following RCTI when compared with contralateral control. Severity of the injury was high in the first two weeks with improvement in 3-4 weeks following RCTI, and this correlated with the healing response. The expression of proteins associated with increased HMGB-1 and upregulation of NLRP3 inflammasome pathway, TLR4, TLR2, TREM-1, RAGE, ASC, Caspase-1, and IL-1β, in the first two weeks following RCTI followed by decline in 3-4 weeks. These results suggest the association of inflammatory responses and ECM disorganization with HMGB1 upregulation and NLRP3 inflammasome activation in the RC tendons and could provide novel target(s) for development of better therapeutic strategies in the management of RCTI.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Trem1  (triggering receptor expressed on myeloid cells 1)

Genes (Mus musculus)
Trem1  (triggering receptor expressed on myeloid cells 1)

Genes (Homo sapiens)
TREM1  (triggering receptor expressed on myeloid cells 1)


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