RGD Reference Report - The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway. - Rat Genome Database

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The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway.

Authors: Chen, Jian Xia  Yi, Xi Juan  Gu, Pei Ling  Gao, Shan Xia 
Citation: Chen JX, etal., Braz J Med Biol Res. 2019 Oct 7;52(10):e8324. doi: 10.1590/1414-431X20198324. eCollection 2019.
RGD ID: 126925216
Pubmed: (View Article at PubMed) PMID:31596310
DOI: Full-text: DOI:10.1590/1414-431X20198324

The aim of this study was to investigate the role of kinase-insert domain-containing receptor (KDR) in intrauterine adhesions (IUA) and its mechanism. The Case group consisted of 92 patients diagnosed with IUA, and the Control group included 86 patients with uterine septum who had normal endometrium verified with an uteroscope. In addition, 50 rats were randomly assigned into Control, Sham, Model, NC-siRNA, and KDR-siRNA groups. Rats in the Model, NC-siRNA, and KDR-siRNA groups were induced by uterine curettage and lipopolysaccharide (LPS) treatment to establish the IUA model. Then, immunohistochemistry was applied for detection of VEGF and KDR expression, HE staining was used for observation of the endometrial morphology and gland counting, Masson staining for measurement of the degree of endometrial fibrosis, and qRT-PCR and western blot for the expression of KDR, VEGF, MMP-9, as well as TGF-β1/Smads pathway-related proteins. Compared with the Control group, the mRNA and protein expressions of KDR were significantly higher in IUA endometrial tissues, and the expression of KDR was positively correlated to the severity of IUA. In addition, the injection of si-KDR increased the number of endometrial glands, reduced the area of fibrosis, inhibited mRNA and protein expression of KDR and VEGF, up-regulated the expression of MMP-9 and Smad7, and decreased the expression level of TGF-β1, p-Smad2, p-Smad3, and Smad4 in rats with IUA. Highly-expressed KDR was related to patients' severity of IUA, and silencing KDR may prevent the occurrence and development of IUA via TGF-β1/Smads signaling pathway and up-regulating the expression of MMP-9.

Annotation

Disease Annotations    
adhesions of uterus  (IEP,IMP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Kdr  (kinase insert domain receptor)
Vegfa  (vascular endothelial growth factor A)

Genes (Mus musculus)
Kdr  (kinase insert domain protein receptor)
Vegfa  (vascular endothelial growth factor A)

Genes (Homo sapiens)
KDR  (kinase insert domain receptor)
VEGFA  (vascular endothelial growth factor A)


Additional Information