RGD Reference Report - Interleukin-27 expression following infection with the murine gammaherpesvirus 68. - Rat Genome Database

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Interleukin-27 expression following infection with the murine gammaherpesvirus 68.

Authors: Nelson, Daniel A  Tolbert, Melanie D  Clemens, Mark G  Bost, Kenneth L 
Citation: Nelson DA, etal., Cytokine. 2010 Aug;51(2):184-94. doi: 10.1016/j.cyto.2010.04.015. Epub 2010 May 20.
RGD ID: 126790537
Pubmed: PMID:20493722   (View Abstract at PubMed)
DOI: DOI:10.1016/j.cyto.2010.04.015   (Journal Full-text)

IL-27 is a heterodimeric cytokine composed of p28 and Epstein Barr virus induced gene 3 (Ebi3) protein subunits. In the present study, we questioned whether murine gammaherpesvirus 68 (HV-68) could induce expression of Ebi3, p28, and IL-27 in this mouse model of an EBV-like infection. Cultured macrophages and dendritic cells exposed to HV-68 upregulated p28 mRNA expression and increased secretion of the p28 and IL-27 (p28+Ebi3) proteins. B220(+) and CD11b(+) cells also upregulated p28 mRNA expression following in vivo infection with this virus. Surprisingly, no significant increases in p28 or IL-27 protein production were observed in vivo during the acute or mononucleosis phases of the disease. The possibility that HV-68-induced upregulation of p28 mRNA expression primed cells for IL-27 secretion was suggested by the ability of a TLR4 agonist to augment cytokine production. When cultured macrophages and dendritic cells were exposed to virus plus a suboptimal dose of LPS, increased levels of p28 protein expression were observed. More importantly, when latently infected mice were challenged with a sublethal dose of LPS, augmented p28 and IL-27 protein production occurred. Using a model of sepsis, mice latently infected with HV-68 had exaggerated p28 protein production when compared to mice that were singularly infected or subjected to cecal ligation and puncture. Taken together, these studies define expression of HV-68 induced IL-27, and suggest that mice latently infected with this gammaherpesvirus will have exaggerated responses when confronted with other stimuli capable of inducing this member of the IL-12 family of cytokines.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL27HumanSepsis  ISOIl27 (Mus musculus)associated with Herpesviridae Infections more ...RGD 
Il27RatSepsis  ISOIl27 (Mus musculus)associated with Herpesviridae Infections more ...RGD 
Il27MouseSepsis  IEP associated with Herpesviridae Infections more ...RGD 

Objects Annotated

Genes (Rattus norvegicus)
Il27  (interleukin 27)

Genes (Mus musculus)
Il27  (interleukin 27)

Genes (Homo sapiens)
IL27  (interleukin 27)


Additional Information